Laurdan in live cell imaging: Effect of acquisition settings, cell culture conditions and data analysis on generalized polarization measurements

Cell perform is extremely depending on membrane construction, group, and fluidity. Due to this fact, strategies to probe the biophysical properties of organic membranes are required. Willpower of generalized polarization (GP) values utilizing Laurdan in fluorescence microscopy research is likely one of the most widely-used strategies to research modifications in membrane fluidity in vitro and…

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bt3technologies

Toward Biodegradable Electronics: Ionic Diodes Based on a Cellulose Nanocrystal-Agarose Hydrogel

Bioderived cellulose nanocrystals (CNCs) are used to create mild, versatile, biocompatible, and biodegradable digital gadgets. Herein, floor modification of cellulose nanocrystals was employed to manufacture cationic and anionic CNCs. Subsequently, we demonstrated rectification conduct from a set junction between two agarose hydrogels doped with cationic and anionic cellulose nanocrystals. The present rectification ratio reaches 70…

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bt3technologies

Activated-carbon-filled agarose hydrogel as a natural medium for seed germination and seedling growth

An activated-carbon-filled agarose (Agar-AC) hydrogel containing numerous concentrations of activated carbon (AC) was efficiently fabricated by a easy solvent solid approach. In comparison with pure agarose hydrogels, Agar-AC hydrogels exhibited glorious mechanical properties, good thermal stability and a extremely developed pore construction. The Agar-AC hydrogels additionally confirmed a sure diploma of enchancment in water retention…

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bt3technologies

Ameliorating quercetin constraints in cancer therapy with pH-responsive agarose-polyvinylpyrrolidone -hydroxyapatite nanocomposite encapsulated in double nanoemulsion

Regardless of quercetin (QC) promising options for most cancers remedy, low solubility, poor permeability, and brief organic half-life time considerably confine its utility in most cancers remedy. On this examine, a novel method is developed to enhance loading effectivity and attain quercetin sustained-release concurrently. On this course, hydrogel nanocomposite of agarose (AG)-polyvinylpyrrolidone (PVP)-hydroxyapatite (HAp) was…

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bt3technologies

Photothermal Agarose Microfabrication Technology for Collective Cell Migration Analysis

Agarose photothermal microfabrication know-how is among the micropatterning methods that has the benefit of easy and versatile real-time fabrication even in the course of the cultivation of cells. To look at the flexibility and limitation of the agarose microstructures, we investigated the collective epithelial cell migration habits in two-dimensional agarose confined buildings. Agarose microchannels from…

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