Tag: mouse spider
Characterization of Tissue Engineered Endothelial Cell Networks in Composite Collagen-Agarose Hydrogels
Scaffolds represent an necessary aspect in vascularized tissues and are subsequently investigated for offering the specified mechanical stability and enabling vasculogenesis and angiogenesis. On this examine, supplementation of hydrogels containing both MatrigelTM and rat tail collagen I (MatrigelTM/rCOL) or human collagen (hCOL) with SeaPlaqueTM agarose had been analyzed with regard to assemble thickness and formation and traits…
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A terminal alkyne and disulfide functionalized agarose resin specifically enriches azidohomoalanine labeled nascent proteins
Nascent proteome presents dynamic modifications in response to a sure stimulus. Thus, monitoring nascent proteome is important to uncovering the concerned organic mechanism. However the low-abundance of nascent proteome towards an amazing pre-existing proteome limits its identification and quantification. Herein, we current a novel technique to complement nascent proteome from entire cell lysate for additional…
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Effects of Mechanical Compression on Chondrogenesis of Human Synovium-Derived Mesenchymal Stem Cells in Agarose Hydrogel
Mechanical compression is a double-edged sword for cartilage transforming, and the impact of mechanical compression on chondrogenic differentiation nonetheless stays elusive so far. Herein, we examine the impact of mechanical dynamic compression on the chondrogenic differentiation of human synovium-derived mesenchymal stem cells (SMSCs). To this goal, SMSCs encapsulated in agarose hydrogels had been cultured in…
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