Tag: elisa araya
Laurdan in live cell imaging: Effect of acquisition settings, cell culture conditions and data analysis on generalized polarization measurements
Cell perform is extremely depending on membrane construction, group, and fluidity. Due to this fact, strategies to probe the biophysical properties of organic membranes are required. Willpower of generalized polarization (GP) values utilizing Laurdan in fluorescence microscopy research is likely one of the most widely-used strategies to research modifications in membrane fluidity in vitro and…
Read MoreHigh-throughput analysis of the steroid profile in placental cell cultures to evaluate endocrine disrupting effects of contaminant exposure
Human placental JEG-Three cells preserve a excessive P450 aromatase exercise and are subsequently appropriate to judge how contaminants could intrude with the routes concerned in estrogen synthesis throughout being pregnant. This has been historically assessed by measuring aromatase exercise by means of the quantity of tritiated water (3H2O) shaped in the course of the aromatization…
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