bt3technologies

How the Supramolecular Nature of Lignohumate Affects Its Diffusion in Agarose Hydrogel

Lignohumate, as an industrially produced analog of pure humic substances, is studied from the standpoint of its diffusion properties. This work focuses on its permeation capacity, necessary in agricultural and horticultural functions, related with its penetration into plant organs as leaves and roots. The hydrogel primarily based on agarose was used as a mannequin materials…

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bt3technologies

In vitro maturation on ovarian granulosa cells encapsulated in agarose matrix improves developmental competence of porcine oocytes

In vivo, mammalian oocytes are surrounded by granulosa cells (GCs) that exist in a three-dimensional (3D) microenvironment with gentle stiffness. The GCs play an necessary function for the in vivo development and growth of oocytes, via bidirectional communication between oocytes and GCs. To imitate the mobile microenvironment of a 3D organized follicle, this examine designed…

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bt3technologies

Effect of Tris Buffer in the Intensity of the Multipoint Covalent Immobilization of Enzymes in Glyoxyl-Agarose Beads

Tris is an extensively used buffer that presents a main amine group on its construction. Within the current work trypsin, chymotrypsin and penicillin G acylase (PGA) had been immobilized/stabilized on glyoxyl agarose in presence of various concentrations of Tris (from zero to 20 mM). The consequences of the presence of Tris throughout immobilization had been…

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bt3technologies

Convenient Agarose Preparation with Hydrogen Peroxide and Desulfation Process Analysis

Agarose is a pure seaweed polysaccharide and broadly used within the medication, meals, and organic fields due to its excessive gel power, non-toxicity, and electrical neutrality. The sulfate group is without doubt one of the essential charged teams that have an effect on the efficiency of agarose. Within the current examine, a easy, eco-friendly, and…

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