A terminal alkyne and disulfide functionalized agarose resin specifically enriches azidohomoalanine labeled nascent proteins

A terminal alkyne and disulfide functionalized agarose resin specifically enriches azidohomoalanine labeled nascent proteins

Nascent proteome presents dynamic adjustments in response to a sure stimulus. Thus, monitoring nascent proteome is important to uncovering the concerned organic mechanism. However the low-abundance of nascent proteome towards an amazing pre-existing proteome limits its identification and quantification. Herein, we current a novel technique to counterpoint nascent proteome from complete cell lysate for additional…

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