Laurdan in live cell imaging: Effect of acquisition settings, cell culture conditions and data analysis on generalized polarization measurements

• Matthew Phillips

Cell perform is extremely depending on membrane construction, group, and fluidity. Due to this fact, strategies to probe the biophysical properties of organic membranes are required. Willpower of generalized polarization (GP) values utilizing Laurdan in fluorescence microscopy research is likely one of the most widely-used strategies to research modifications in membrane fluidity in vitro and…