bt3technologies

Stepwise neuronal network pattern formation in agarose gel during cultivation using non-destructive microneedle photothermal microfabrication

Typical neuronal community sample formation strategies can not management the association of axons and dendrites as a result of community buildings have to be fastened earlier than neurite differentiation. To beat this limitation, we developed a non-destructive stepwise microfabrication approach that can be utilized to change microchannels inside agarose to information neurites throughout elongation.
Micropatterns have been fashioned in skinny agarose layer coating of a cultivation dish utilizing the tip of a 0.7 [Formula: see text]-diameter platinum-coated glass microneedle heated by a centered 1064-nm wavelength infrared laser, which has no absorbance of water. As the scale of the warmth supply was 0.7 [Formula: see text], which is smaller than the laser wavelength, the temperature fell to 45 [Formula: see text] inside a distance of seven.0 [Formula: see text] from the sting of the etched agarose microchannel.
We exploited the quick temperature decay property to information cell-to-cell connection throughout neuronal community cultivation. The primary neurite of a hippocampal cell from a microchamber was guided to a microchannel resulting in the goal neuron with stepwise etching of the micrometer decision microchannel within the agarose layer, and the elongated neurites weren’t broken by the warmth of etching. The outcomes point out the potential of this new approach for totally direction-controlled on-chip neuronal community research.

Synthesis of C6-amino agarose and analysis of its antibacterial exercise

On this paper, the biologically inert agarose was selectively modified at C6 of β-d-Galp items to supply an amino spinoff with antibacterial property. The artificial route concerned the preparation of tosyl and azido agarose intermediates. All of the polysaccharide derivatives have been characterised by mono- and bidimensional 1H and 13C NMR and FT-IR evaluation. A water-soluble amino polymer (Mw = 39,000 g mol-1, DSamino = 0.50) was produced by partial acid hydrolysis displaying bactericidal and bacteriostatic exercise in opposition to P. aeruginosa (ATCC 9027), S. aureus (ATCC 6538), and E. coli (ATCC 25922), with MIC values decrease than 2.5 mg mL-1 and MBC values starting from 2.5 to five.Zero mg mL-1.
Varied well being helpful outcomes related to purple seaweeds, particularly their polysaccharides, have been claimed, however the molecular pathway of how purple seaweed polysaccharides are degraded and utilized by cooperative actions of human intestine micro organism has not been elucidated.
  • Right here, we investigated the enzymatic and metabolic cooperation between two human intestine symbionts, Bacteroides plebeius and Bifidobacterium longum ssp. infantis, with regard to the degradation of agarose, the primary carbohydrate of purple seaweed.
  • Extra particularly, B. plebeius initially decomposed agarose into agarotriose by the actions of the enzymes belonging to glycoside hydrolase (GH) households 16 and 117 (i.e., BpGH16A and BpGH117) situated within the polysaccharide utilization locus, a selected gene cluster for purple seaweed carbohydrates.
  • Then, B. infantis extracted power from agarotriose by the actions of two agarolytic β-galactosidases (i.e., Bga42A and Bga2A) and produced neoagarobiose. B. plebeius in the end acted on neoagarobiose by BpGH117, ensuing within the manufacturing of three,6-anhydro-L-galactose, a monomeric sugar possessing anti-inflammatory exercise.
  • Our discovery of the cooperative actions of the 2 human intestine symbionts on agarose degradation and the identification of the associated enzyme genes and metabolic intermediates generated in the course of the metabolic processes present a molecular foundation for agarose degradation by intestine micro organism.
Human dental follicle cells (HDFCs) are a really perfect cell supply of stem cells for dental tissue restore and regeneration and so they have nice potential for regenerative drugs functions. Nevertheless, the standard monolayer tradition normally reduces cell proliferation and differentiation potential because of the steady passage throughout in vitro enlargement. On this examine, major HDFC spheroids have been generated on 1% agarose, and the HDFCs spontaneously fashioned cell spheroids within the agarose-coated dishes.
In contrast with monolayer tradition, the spheroid-derived HDFCs exhibited elevated proliferative capability for later passage HDFCs as analysed by Cell Counting Equipment-8 (CCK-8). The transcription-quantitative polymerase chain response (qRT-PCR), western blot and immunofluorescence assay confirmed that the expression of stemness marker genes Sox2, Oct4 and Nanog was elevated considerably within the HDFC spheroids. Moreover, we discovered that the odontogenic differentiation functionality of HDFCs was considerably improved by spheroid tradition within the agarose-coated dishes.
However, the osteogenic differentiation functionality was weakened in contrast with monolayer tradition. Our outcomes recommend that spheroid formation of HDFCs in agarose-coated dishes partially restores the proliferative capability of HDFCs at later passages, enhances their stemness and improves odontogenic differentiation functionality in vitro. Due to this fact, spheroid formation of HDFCs has nice therapeutic potential for stem cell medical remedy.
bt3technologies
bt3technologies

Agarose Stearate-Carbomer 940 as Stabilizer and Rheology Modifier for Surfactant-Free Beauty Formulations

 

Some generally used surfactants in beauty merchandise elevate considerations attributable to their skin-irritating results and environmental contamination. Multifunctional, high-performance polymers are good alternate options to beat these issues. On this examine, agarose stearate (AS) with emulsifying, thickening, and gel properties was synthesized. Surfactant-free beauty formulations have been efficiently ready from AS and carbomer940 (CBM940) combined programs. The correlation of rheological parameter with pores and skin feeling was decided to review the usability of the combined programs in cosmetics.
Based mostly on rheological evaluation, the surfactant-free beauty cream (SFC) stabilized by AS-carbomer940 confirmed shear-thinning conduct and strongly synergistic motion. The SFC exhibited a gel-like conduct and had rheological properties just like business beauty lotions. Scanning electron microscope photographs proved that the AS-CBM940 community performed an essential position in SFC’s stability. Oil content material might reinforce the elastic traits of the AS-CBM940 matrix.
The SFCs confirmed look and sensation throughout and after rubbing into pores and skin. The information gained from this examine could also be helpful for designing surfactant-free beauty cream with rheological properties that may be tailor-made for specific business beauty functions. They could even be helpful for producing drugs merchandise with extremely viscous or gel-like textures, reminiscent of some ointments and wound dressings.

FAM FLICA CASPASE-3/7 KIT (OKSA11271)

OKSA11271 100 Tests
EUR 1524
Description: Description of target: The FAM FLICA™ Caspase 3 & 7 kit uses a quick and easy method to analyze active caspases in apoptotic cells.;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

Caspase 6 Assay Kit

20-abx299018
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

FAM FLICAâ„¢ CASPASE-6 KIT (OKSA11272)

OKSA11272 25 Tests
EUR 598
Description: Description of target: FAM FLICAâ„¢ CASPASE-6 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ CASPASE-6 KIT (OKSA11273)

OKSA11273 100 Tests
EUR 1550
Description: Description of target: FAM FLICAâ„¢ CASPASE-6 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

Caspase 6 Assay Kit, green

KF17204 25 Tests
EUR 304

Caspase-6 Fluorometric Assay Kit

K2014-200 200 assays
EUR 696

Caspase-6 Fluorometric Assay Kit

K2014-25 25 assays
EUR 238

Caspase-6 Fluorometric Assay Kit

K2014-400 400 assays
EUR 1086

Caspase-6 Colorimetric Assay Kit

K2015-200 200 assays
EUR 696

Caspase-6 Colorimetric Assay Kit

K2015-25 25 assays
EUR 238

Caspase-6 Colorimetric Assay Kit

K2015-400 400 assays
EUR 1101

Caspase 6 Fluorometric Assay Kit

55R-1276 25 assays
EUR 287
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 6 Colorimetric Assay Kit

55R-1277 25 assays
EUR 284
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase-6 Fluorometric Assay Kit

K114-100
EUR 430

Caspase-6 Fluorometric Assay Kit

K114-200
EUR 615

Caspase-6 Fluorometric Assay Kit

K114-25
EUR 202

Caspase-6 Fluorometric Assay Kit

K114-400
EUR 958

Caspase-6 Colorimetric Assay Kit

K115-100
EUR 452

Caspase-6 Colorimetric Assay Kit

K115-200
EUR 615

Caspase-6 Colorimetric Assay Kit

K115-25
EUR 202

Caspase-6 Colorimetric Assay Kit

K115-400
EUR 958

6-FAM

HY-66021 500mg
EUR 312

Genorise® Caspase-6 Colorimetric Assay Kit

GR107023 100 tests
EUR 289

Genorise® Caspase-6 Fluorometric Assay Kit

GR107024 100 tests
EUR 289

Caspase 6 Colorimetric Cell-Based ELISA Kit

EKC1087 100ul
EUR 572

6-FAM Azide

955 100 mg
EUR 702

6-FAM Alkyne

956 100 mg
EUR 702

6-FAM SE

HY-15939 100mg
EUR 612

6 FAM SE

A8771-10 10 mg
EUR 174
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.

6 FAM SE

A8771-100 100 mg
EUR 731
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.

6 FAM SE

A8771-5.1 10 mM (in 1mL DMSO)
EUR 199
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.

6 FAM SE

A8771-50 50 mg
EUR 453
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.

Caspase 6 Colorimetric Cell-Based ELISA Kit (OKAG00581)

OKAG00581 96 Wells
EUR 596
Description: Description of target: ;Species reactivity: Human, Mouse, Rat;Application: ELISA;Assay info: Assay Type: Cell-Based
Subtype: None
Detection Method: Colorimetric 450 nm;Sensitivity:

FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11268)

OKSA11268 25 Tests
EUR 591
Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11269)

OKSA11269 100 Tests
EUR 1524
Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ CASPASE-8 KIT (OKSA11276)

OKSA11276 25 Tests
EUR 591
Description: Description of target: FAM FLICAâ„¢ CASPASE-8 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ CASPASE-8 KIT (OKSA11277)

OKSA11277 100 Tests
EUR 1524
Description: Description of target: FAM FLICAâ„¢ CASPASE-8 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ CASPASE-9 KIT (OKSA11278)

OKSA11278 25 Tests
EUR 591
Description: Description of target: FAM FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ CASPASE-9 KIT (OKSA11279)

OKSA11279 100 Tests
EUR 1524
Description: Description of target: FAM FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ CASPASE-2 KIT (OKSA11282)

OKSA11282 25 Tests
EUR 557
Description: Description of target: FAM FLICAâ„¢ CASPASE-2 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

Caspase 3 Assay Kit

20-abx294003
  • EUR 1255.00
  • EUR 457.00
  • EUR 770.00
  • 100 tests
  • 20 tests
  • 50 tests

Poly-Caspase Assay Kit

20-abx299016
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 1 Assay Kit

20-abx299019
  • EUR 1365.00
  • EUR 629.00
  • 100 tests
  • 25 tests

Caspase 8 Assay Kit

20-abx299020
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 9 Assay Kit

20-abx299021
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Poly-Caspase Assay Kit

abx299022-2550tests 25-50 tests
EUR 692

Caspase 1 Assay Kit

abx299023-2550tests 25-50 tests
EUR 692

Poly-Caspase Assay Kit

20-abx299025
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 2 Assay Kit

abx299026-25tests 25 tests
EUR 582

Caspase 10 Assay Kit

abx299027-100tests 100 tests
EUR 1302

Caspase 9 Assay Kit

20-abx299035
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 12 Assay Kit

55R-1290 25 assays
EUR 328
Description: Assay Kit for detection of Capase 12 activity in the research laboratory

FAM-Phe-CMK FLISP™ Assay Kit

KF17314 25 Tests
EUR 291

FAM-Phe-CMK FLISP™ Assay Kit

KF17315 100 Tests
EUR 695

FAM-Lue-CMK FLISP™ Assay Kit

KF17316 25 Tests
EUR 291

FAM-Leu-CMK FLISP™ Assay Kit

KF17317 100 Tests
EUR 695

FAM-Phe-CMK Serine Protease Assay Kit

abx299010-25tests 25 tests
EUR 566

FAM-Leu-CMK Serine Protease Assay Kit

abx299011-25tests 25 tests
EUR 566

FAM-Leu-DAP Serine Protease Assay Kit

abx299012-25tests 25 tests
EUR 566

FAM-Phe-DAP Serine Protease Assay Kit

abx299013-25tests 25 tests
EUR 582

5(6)-FAM SE

HY-15937 100mg
EUR 215

Aminooxy-5(6)-FAM

90103 5MG
EUR 242
Description: Minimum order quantity: 1 unit of 5MG

FAM azide, 6- isomer

A8118-25 25 mg
EUR 392
Description: FAM (fluorescein) azide. solid and 10 mM solution in DMSO, labeling reagent for Click chemistry.Pure 6-isomer.

FAM azide, 6- isomer

A8118-5 5 mg
EUR 166
Description: FAM (fluorescein) azide. solid and 10 mM solution in DMSO, labeling reagent for Click chemistry.Pure 6-isomer.

Caspase 6 (Phospho-Ser257) Colorimetric Cell-Based ELISA Kit

EKC1975 100ul
EUR 572

Glycerol Cell-Based Assay Kit

K977-100
EUR 490

6-carboxyfluorescein, se (6-fam, se)

90023 10MG
EUR 191
Description: Minimum order quantity: 1 unit of 10MG

FAM-Spacer-Phe-CMK FLISP™ Assay Kit

KF17322 25 Tests
EUR 291

FAM-Spacer-Phe-CMK FLISP™ Assay Kit

KF17323 100 Tests
EUR 695

FAM-Spacer-Leu-CMK FLISP™ Assay Kit

KF17324 25 Tests
EUR 291