2 Apb 4-Hne Antibody Anti-Ubiquitin Antibody Arf1 Antibody azide azide free Cdc20 Antibody Influenza Kd Antibody Kdel Antibody Lizard Macaw Mettl3 Antibody Mink Mouse Opossum Rhcg Antibody Tyrosinase Antibody
Stepwise neuronal network pattern formation in agarose gel during cultivation using non-destructive microneedle photothermal microfabrication
Typical neuronal community sample formation strategies can not management the association of axons and dendrites as a result of community buildings have to be fastened earlier than neurite differentiation. To beat this limitation, we developed a non-destructive stepwise microfabrication approach that can be utilized to change microchannels inside agarose to information neurites throughout elongation.
Micropatterns have been fashioned in skinny agarose layer coating of a cultivation dish utilizing the tip of a 0.7 [Formula: see text]-diameter platinum-coated glass microneedle heated by a centered 1064-nm wavelength infrared laser, which has no absorbance of water. As the scale of the warmth supply was 0.7 [Formula: see text], which is smaller than the laser wavelength, the temperature fell to 45 [Formula: see text] inside a distance of seven.0 [Formula: see text] from the sting of the etched agarose microchannel.
We exploited the quick temperature decay property to information cell-to-cell connection throughout neuronal community cultivation. The primary neurite of a hippocampal cell from a microchamber was guided to a microchannel resulting in the goal neuron with stepwise etching of the micrometer decision microchannel within the agarose layer, and the elongated neurites weren’t broken by the warmth of etching. The outcomes point out the potential of this new approach for totally direction-controlled on-chip neuronal community research.
Physics of agarose fluid gels: Rheological properties and microstructure
Agarose, a strongly gelling polysaccharide, is a standard ingredient used to optimize the viscoelastic properties of a mess of meals merchandise. By way of aggregation of double helices through hydrogen bonds whereas cooling underneath quiescent situations it types agency and brittle gels. Nevertheless, this conduct could be altered by manipulating the processing situations viz shear. For instance, gelation underneath shear results in microgel particles with massive floor space, which in flip results in fully totally different rheological properties and texture. Such fluid gels are proven to play an essential position in texture modification of meals and drinks for dysphagia sufferers.
On this examine, totally different focus of agarose fluid gel (0.5 % wt, 1 % wt and a pair of % wt) have been thought-about. Rheological measurements of the microgel particles confirmed a rise of storage and loss modulus with rising focus. Nevertheless, 1 % wt fluid gel exhibited the bottom viscosity within the low shear vary and the shortest LVE vary. Moreover, the impact on the microstructure and measurement of gel particles have been additionally investigated through the use of mild microscopy and particle measurement evaluation.
It was noticed that because the focus of agarose elevated the particle measurement and unordered chains current on the particle floor decreases. Based mostly on our outcomes, we suggest particular fashions suggesting the impression of the particle measurement, the focus and the “furry” projections on the rheological and tribological properties that might assist in understanding the variations in traits of fluid gels.
Synthesis of C6-amino agarose and analysis of its antibacterial exercise
On this paper, the biologically inert agarose was selectively modified at C6 of β-d-Galp items to supply an amino spinoff with antibacterial property. The artificial route concerned the preparation of tosyl and azido agarose intermediates. All of the polysaccharide derivatives have been characterised by mono- and bidimensional 1H and 13C NMR and FT-IR evaluation. A water-soluble amino polymer (Mw = 39,000 g mol-1, DSamino = 0.50) was produced by partial acid hydrolysis displaying bactericidal and bacteriostatic exercise in opposition to P. aeruginosa (ATCC 9027), S. aureus (ATCC 6538), and E. coli (ATCC 25922), with MIC values decrease than 2.5 mg mL-1 and MBC values starting from 2.5 to five.Zero mg mL-1.
Varied well being helpful outcomes related to purple seaweeds, particularly their polysaccharides, have been claimed, however the molecular pathway of how purple seaweed polysaccharides are degraded and utilized by cooperative actions of human intestine micro organism has not been elucidated.
- Right here, we investigated the enzymatic and metabolic cooperation between two human intestine symbionts, Bacteroides plebeius and Bifidobacterium longum ssp. infantis, with regard to the degradation of agarose, the primary carbohydrate of purple seaweed.
- Extra particularly, B. plebeius initially decomposed agarose into agarotriose by the actions of the enzymes belonging to glycoside hydrolase (GH) households 16 and 117 (i.e., BpGH16A and BpGH117) situated within the polysaccharide utilization locus, a selected gene cluster for purple seaweed carbohydrates.
- Then, B. infantis extracted power from agarotriose by the actions of two agarolytic β-galactosidases (i.e., Bga42A and Bga2A) and produced neoagarobiose. B. plebeius in the end acted on neoagarobiose by BpGH117, ensuing within the manufacturing of three,6-anhydro-L-galactose, a monomeric sugar possessing anti-inflammatory exercise.
- Our discovery of the cooperative actions of the 2 human intestine symbionts on agarose degradation and the identification of the associated enzyme genes and metabolic intermediates generated in the course of the metabolic processes present a molecular foundation for agarose degradation by intestine micro organism.
Agarose-based spheroid tradition enhanced stemness and promoted odontogenic differentiation potential of human dental follicle cells in vitro
Human dental follicle cells (HDFCs) are a really perfect cell supply of stem cells for dental tissue restore and regeneration and so they have nice potential for regenerative drugs functions. Nevertheless, the standard monolayer tradition normally reduces cell proliferation and differentiation potential because of the steady passage throughout in vitro enlargement. On this examine, major HDFC spheroids have been generated on 1% agarose, and the HDFCs spontaneously fashioned cell spheroids within the agarose-coated dishes.
In contrast with monolayer tradition, the spheroid-derived HDFCs exhibited elevated proliferative capability for later passage HDFCs as analysed by Cell Counting Equipment-8 (CCK-8). The transcription-quantitative polymerase chain response (qRT-PCR), western blot and immunofluorescence assay confirmed that the expression of stemness marker genes Sox2, Oct4 and Nanog was elevated considerably within the HDFC spheroids. Moreover, we discovered that the odontogenic differentiation functionality of HDFCs was considerably improved by spheroid tradition within the agarose-coated dishes.
However, the osteogenic differentiation functionality was weakened in contrast with monolayer tradition. Our outcomes recommend that spheroid formation of HDFCs in agarose-coated dishes partially restores the proliferative capability of HDFCs at later passages, enhances their stemness and improves odontogenic differentiation functionality in vitro. Due to this fact, spheroid formation of HDFCs has nice therapeutic potential for stem cell medical remedy.
Agarose Stearate-Carbomer 940 as Stabilizer and Rheology Modifier for Surfactant-Free Beauty Formulations
Some generally used surfactants in beauty merchandise elevate considerations attributable to their skin-irritating results and environmental contamination. Multifunctional, high-performance polymers are good alternate options to beat these issues. On this examine, agarose stearate (AS) with emulsifying, thickening, and gel properties was synthesized. Surfactant-free beauty formulations have been efficiently ready from AS and carbomer940 (CBM940) combined programs. The correlation of rheological parameter with pores and skin feeling was decided to review the usability of the combined programs in cosmetics.
Based mostly on rheological evaluation, the surfactant-free beauty cream (SFC) stabilized by AS-carbomer940 confirmed shear-thinning conduct and strongly synergistic motion. The SFC exhibited a gel-like conduct and had rheological properties just like business beauty lotions. Scanning electron microscope photographs proved that the AS-CBM940 community performed an essential position in SFC’s stability. Oil content material might reinforce the elastic traits of the AS-CBM940 matrix.
The SFCs confirmed look and sensation throughout and after rubbing into pores and skin. The information gained from this examine could also be helpful for designing surfactant-free beauty cream with rheological properties that may be tailor-made for specific business beauty functions. They could even be helpful for producing drugs merchandise with extremely viscous or gel-like textures, reminiscent of some ointments and wound dressings.
) FAM FLICA CASPASE-3/7 KIT (OKSA11271) |
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| OKSA11271 | Aviva Systems Biology | 100 Tests | EUR 1828.8 |
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Description: Description of target: The FAM FLICA™ Caspase 3 & 7 kit uses a quick and easy method to analyze active caspases in apoptotic cells.;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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) FAM FLICAâ„¢ CASPASE-6 KIT (OKSA11272) |
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| OKSA11272 | Aviva Systems Biology | 25 Tests | EUR 717.6 |
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Description: Description of target: FAM FLICAâ„¢ CASPASE-6 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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) FAM FLICAâ„¢ CASPASE-6 KIT (OKSA11273) |
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| OKSA11273 | Aviva Systems Biology | 100 Tests | EUR 1860 |
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Description: Description of target: FAM FLICAâ„¢ CASPASE-6 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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 Caspase 6 Assay Kit |
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| 20-abx299018 | Abbexa |
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 Caspase 6 Fluorometric Assay Kit |
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| 55R-1276 | Fitzgerald | 25 assays | EUR 344.4 |
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Description: Assay Kit for detection of Capase 6 activity in the research laboratory |
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 Caspase 6 Colorimetric Assay Kit |
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| 55R-1277 | Fitzgerald | 25 assays | EUR 340.8 |
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Description: Assay Kit for detection of Capase 6 activity in the research laboratory |
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 Caspase 6 Assay Kit, green |
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| KF17204 | Neuromics | 25 Tests | EUR 364.8 |
 Caspase-6 Fluorometric Assay Kit |
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| K2014-200 | ApexBio | 200 assays | EUR 835.2 |
Caspase-6 Fluorometric Assay Kit |
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| K2014-25 | ApexBio | 25 assays | EUR 285.6 |
Caspase-6 Fluorometric Assay Kit |
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| K2014-400 | ApexBio | 400 assays | EUR 1303.2 |
 Caspase-6 Colorimetric Assay Kit |
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| K2015-200 | ApexBio | 200 assays | EUR 835.2 |
Caspase-6 Colorimetric Assay Kit |
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| K2015-25 | ApexBio | 25 assays | EUR 285.6 |
Caspase-6 Colorimetric Assay Kit |
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| K2015-400 | ApexBio | 400 assays | EUR 1321.2 |
Caspase-6 Fluorometric Assay Kit |
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| K114-100 | Biovision | each | EUR 516 |
Caspase-6 Fluorometric Assay Kit |
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| K114-200 | Biovision | each | EUR 738 |
Caspase-6 Fluorometric Assay Kit |
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| K114-25 | Biovision | each | EUR 242.4 |
Caspase-6 Fluorometric Assay Kit |
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| K114-400 | Biovision | each | EUR 1149.6 |
Caspase-6 Colorimetric Assay Kit |
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| K115-100 | Biovision | each | EUR 542.4 |
Caspase-6 Colorimetric Assay Kit |
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| K115-200 | Biovision | each | EUR 738 |
Caspase-6 Colorimetric Assay Kit |
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| K115-25 | Biovision | each | EUR 242.4 |
Caspase-6 Colorimetric Assay Kit |
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| K115-400 | Biovision | each | EUR 1149.6 |
 Caspase-6 Homogeneous Assay Kit |
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| 80703 | BPS Bioscience | 96 rxns. | EUR 385 |
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Description: The Homogeneous Caspase-6 Assay Kit is a complete assay system designed to measure Caspase-6 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-6 activity measurements. In addition, the kit includes purified Caspase-6 enzyme and a potent Caspase-6 inhibitor, Ac-IETD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-6 activity level. The fluorogenic substrate, Ac-VEID-AFC, is incubated with purified Caspase-6 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader. |
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 6-FAM |
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| HY-66021 | MedChemExpress | 500mg | EUR 374.4 |
 Caspase 6 Colorimetric Cell-Based ELISA Kit |
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| EKC1087 | BosterBio | 100ul | EUR 686.4 |
 6 FAM SE |
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| A8771-10 | ApexBio | 10 mg | EUR 208.8 |
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Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein. |
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6 FAM SE |
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| A8771-100 | ApexBio | 100 mg | EUR 877.2 |
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Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein. |
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6 FAM SE |
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| A8771-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 238.8 |
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Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein. |
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6 FAM SE |
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| A8771-50 | ApexBio | 50 mg | EUR 543.6 |
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Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein. |
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 6-FAM Azide |
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| 955 | AAT Bioquest | 100 mg | EUR 842.4 |
 6-FAM Alkyne |
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| 956 | AAT Bioquest | 100 mg | EUR 842.4 |
 6-FAM SE |
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| HY-15939 | MedChemExpress | 100mg | EUR 734.4 |
 Genorise® Caspase-6 Colorimetric Assay Kit |
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| GR107023 | Genorise Scientific | 100 tests | EUR 346.8 |
 Genorise® Caspase-6 Fluorometric Assay Kit |
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| GR107024 | Genorise Scientific | 100 tests | EUR 346.8 |
) FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11268) |
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| OKSA11268 | Aviva Systems Biology | 25 Tests | EUR 709.2 |
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Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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) FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11269) |
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| OKSA11269 | Aviva Systems Biology | 100 Tests | EUR 1828.8 |
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Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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) FAM FLICAâ„¢ CASPASE-8 KIT (OKSA11276) |
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| OKSA11276 | Aviva Systems Biology | 25 Tests | EUR 709.2 |
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Description: Description of target: FAM FLICAâ„¢ CASPASE-8 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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) FAM FLICAâ„¢ CASPASE-8 KIT (OKSA11277) |
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| OKSA11277 | Aviva Systems Biology | 100 Tests | EUR 1828.8 |
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Description: Description of target: FAM FLICAâ„¢ CASPASE-8 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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) FAM FLICAâ„¢ CASPASE-9 KIT (OKSA11278) |
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| OKSA11278 | Aviva Systems Biology | 25 Tests | EUR 709.2 |
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Description: Description of target: FAM FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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) FAM FLICAâ„¢ CASPASE-9 KIT (OKSA11279) |
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| OKSA11279 | Aviva Systems Biology | 100 Tests | EUR 1828.8 |
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Description: Description of target: FAM FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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) FAM FLICAâ„¢ CASPASE-2 KIT (OKSA11282) |
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| OKSA11282 | Aviva Systems Biology | 25 Tests | EUR 668.4 |
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Description: Description of target: FAM FLICAâ„¢ CASPASE-2 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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) Caspase 6 Colorimetric Cell-Based ELISA Kit (OKAG00581) |
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| OKAG00581 | Aviva Systems Biology | 96 Wells | EUR 715.2 |
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Description: Description of target: ;Species reactivity: Human, Mouse, Rat;Application: ELISA;Assay info: Assay Type: Cell-Based Subtype: None Detection Method: Colorimetric 450 nm;Sensitivity: |
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 FAM azide, 6- isomer |
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| A8118-25 | ApexBio | 25 mg | EUR 470.4 |
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Description: FAM (fluorescein) azide. solid and 10 mM solution in DMSO, labeling reagent for Click chemistry.Pure 6-isomer. |
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FAM azide, 6- isomer |
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| A8118-5 | ApexBio | 5 mg | EUR 199.2 |
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Description: FAM (fluorescein) azide. solid and 10 mM solution in DMSO, labeling reagent for Click chemistry.Pure 6-isomer. |
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-FAM) Aminooxy-5(6)-FAM |
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| 90103 | Biotium | 5MG | EUR 290.4 |
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Description: Minimum order quantity: 1 unit of 5MG |
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-FAM SE) 5(6)-FAM SE |
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| HY-15937 | MedChemExpress | 100mg | EUR 258 |
 FAM-Phe-CMK Serine Protease Assay Kit |
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| abx299010-25tests | Abbexa | 25 tests | EUR 679.2 |
 FAM-Leu-CMK Serine Protease Assay Kit |
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| abx299011-25tests | Abbexa | 25 tests | EUR 679.2 |
 FAM-Leu-DAP Serine Protease Assay Kit |
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| abx299012-25tests | Abbexa | 25 tests | EUR 679.2 |
 FAM-Phe-DAP Serine Protease Assay Kit |
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| abx299013-25tests | Abbexa | 25 tests | EUR 698.4 |
 FAM-Phe-CMK FLISP™ Assay Kit |
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| KF17314 | Neuromics | 25 Tests | EUR 349.2 |
FAM-Phe-CMK FLISP™ Assay Kit |
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| KF17315 | Neuromics | 100 Tests | EUR 834 |
 FAM-Lue-CMK FLISP™ Assay Kit |
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| KF17316 | Neuromics | 25 Tests | EUR 349.2 |
 FAM-Leu-CMK FLISP™ Assay Kit |
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| KF17317 | Neuromics | 100 Tests | EUR 834 |
 Colorimetric Cell-Based ELISA Kit) Caspase 6 (Phospho-Ser257) Colorimetric Cell-Based ELISA Kit |
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| EKC1975 | BosterBio | 100ul | EUR 686.4 |
) 6-carboxyfluorescein, se (6-fam, se) |
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| 90023 | Biotium | 10MG | EUR 229.2 |
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Description: Minimum order quantity: 1 unit of 10MG |
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 Caspase 12 Assay Kit |
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| 55R-1290 | Fitzgerald | 25 assays | EUR 393.6 |
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Description: Assay Kit for detection of Capase 12 activity in the research laboratory |
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 Caspase 3 Assay Kit |
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| 20-abx294003 | Abbexa |
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 Poly-Caspase Assay Kit |
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| 20-abx299016 | Abbexa |
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 Caspase 1 Assay Kit |
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| 20-abx299019 | Abbexa |
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 Caspase 8 Assay Kit |
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| 20-abx299020 | Abbexa |
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 Caspase 9 Assay Kit |
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| 20-abx299021 | Abbexa |
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Poly-Caspase Assay Kit |
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| abx299022-2550tests | Abbexa | 25-50 tests | EUR 830.4 |
Caspase 1 Assay Kit |
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| abx299023-2550tests | Abbexa | 25-50 tests | EUR 830.4 |
Poly-Caspase Assay Kit |
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| 20-abx299025 | Abbexa |
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 Caspase 2 Assay Kit |
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| abx299026-25tests | Abbexa | 25 tests | EUR 698.4 |
 Caspase 10 Assay Kit |
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| abx299027-100tests | Abbexa | 100 tests | EUR 1562.4 |
Caspase 9 Assay Kit |
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| 20-abx299035 | Abbexa |
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 Glycerol Cell-Based Assay Kit |
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| K977-100 | Biovision | each | EUR 588 |
 Colorimetric Cell-Based ELISA Kit (OKAG01500)) Phospho-Caspase 6 (Ser257) Colorimetric Cell-Based ELISA Kit (OKAG01500) |
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| OKAG01500 | Aviva Systems Biology | 2 x 96 Wells | EUR 888 |
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Description: Description of target: ;Species reactivity: Human: S257, Mouse: S239, Rat: S240;Application: ELISA;Assay info: Assay Type: Cell-Based Subtype: Phospho Detection Method: Colorimetric 450 nm;Sensitivity: |
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 FAM-Spacer-Phe-CMK FLISP™ Assay Kit |
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| KF17322 | Neuromics | 25 Tests | EUR 349.2 |
