bt3technologies

Photothermal Agarose Microfabrication Technology for Collective Cell Migration Analysis

Agarose photothermal microfabrication know-how is among the micropatterning methods that has the benefit of easy and versatile real-time fabrication even in the course of the cultivation of cells. To look at the flexibility and limitation of the agarose microstructures, we investigated the collective epithelial cell migration habits in two-dimensional agarose confined buildings. Agarose microchannels from 10 to 211 micrometer width had been fabricated with a spot heating of a centered 1480 nm wavelength infrared laser to the skinny agarose layer coated on the cultivation dish after the cells occupied the reservoir.
The collective cell migration velocity maintained fixed no matter their extension distance, whereas the width dependency of these velocities was maximized round 30 micrometer width and decreased each within the narrower and wider microchannels. The only-cell monitoring revealed that the lower of velocity within the narrower width was brought on by the obvious improve of facet ratio of cell form (as much as 8.9). In distinction, the lower within the wider channels was primarily brought on by the rise of the random walk-like habits of part cells. The outcomes confirmed the benefits of this technique: (1) versatile fabrication with none pre-designing, (2) modification even throughout cultivation, and (3) the cells had been confined within the agarose geometry.

Semi-Computerized Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Purposes

 

On this paper, a prototype of a semi-automatic lab-on-PCB for agarose gel preparation and electrophoresis is developed. The scale of the machine are 38 × 34 mm2 and it features a conductivity sensor for detecting the TAE buffer (Tris-acetate-EDTA buffer), a microheater for growing the solubility of the agarose, a unfavorable temperature coefficient (NTC) thermistor for controlling the temperature, a lightweight dependent resistor (LDR) sensor for measuring the transparency of the combination, and two electrodes for performing the electrophoresis. The agarose preparation features are ruled by a microcontroller.
The machine requires a PMMA construction to outline the wells of the agarose gel, and to launch the electrodes from the agarose. The utmost voltage and present that the system requires are 40 V to carry out the electrophoresis, and 1 A for activating the microheater. The chosen temperature for mixing is 80 ∘C, with a mixing time of 10 min. As well as, the curing time is about 30 min. This machine is meant to be built-in as part of a bigger lab-on-PCB system for DNA amplification and detection. Nonetheless, it may be used emigrate DNA amplified in standard thermocyclers. Furthermore, the machine might be modified for getting ready bigger agarose gels and performing electrophoresis.

Novel Thermotolerant Amylase from Bacillus licheniformis Pressure LB04: Purification, Characterization and Agar-Agarose

This examine analyzed the thermostability and impact of calcium ions on the enzymatic exercise of α-amylase produced by Bacillus licheniformis pressure LB04 remoted from Espinazo Scorching springs in Nuevo Leon, Mexico. The enzyme was immobilized by entrapment on agar-agarose beads, with an entrapment yield of 19.9%. The identification of the micro organism was carried out utilizing 16s rDNA sequencing.
The enzyme was purified by way of ion trade chromatography (IEX) in a DEAE-Sephadex column, revealing a protein with a molecular weight of ≈130 kDa. The enzyme was steady at pH 3.Zero and warmth steady as much as 80 °C. Nonetheless, the optimum circumstances had been reached at 65 °C and pH 3.0, with a selected exercise of 1851.7 U mg-1 ± 1.3. The agar-agarose immobilized α-amylase had a hydrolytic exercise almost 25% larger when in comparison with the free enzyme. This examine gives important data for the understanding of the enzymatic profile of B. licheniformis pressure LB04 and the potential utility of the microorganisms at an industrial stage, particularly within the meals business.

Chemotaxis: Underneath Agarose Assay

 

The unicellular eukaryotic amoeba, Dictyostelium discoideum, represents an excellent mannequin for inspecting the molecular mechanism of chemotaxis. Underneath vegetative circumstances, the amoebae are chemotactically conscious of pterins, equivalent to folic acid. Underneath starved circumstances, they lose their sensitivity to pterins and turn out to be chemotactically conscious of cAMP. As an NIH mannequin system, Dictyostelium affords quite a lot of benefits in learning chemotaxis, together with ease of development, genetic tractability, and the conservation of mammalian signaling pathways. On this chapter, we describe the usage of the under-agarose chemotaxis assay to grasp the signaling pathways controlling directional sensing and motility in Dictyostelium discoideum. Given the similarities between Dictyostelium and mammalian cells, this permits us to dissect conserved pathways concerned in eukaryotic chemotaxis.

Expression and Ni-NTA-Agarose Purification of Recombinant Hepatitis C Virus E2Ectodomain Produced in a Baculovirus Expression System

 

On this protocol, we describe the manufacturing and purification of the ectodomain of the E2661 envelope protein (amino acids 384-661) of the Hepatitis C virus, which performs a elementary function within the entry of the virus into the host cell. This protein has been expressed in each prokaryotic and eukaryotic programs however in small portions or with out native protein traits.
  • In our case, we use the Baculovirus expression system in insect cells. E2661 is secreted into the extracellular medium and purified via affinity chromatography a Ni-NTA-column as a result of the protein has a tag of six histidines at its amino terminal finish.
  • The purified protein possesses a native-like conformation and it’s produced in giant portions, round 5-6 mg per liter.
  • Sensible hydrogels conscious of minimally invasive near-infrared (NIR) gentle have nice potential in localized drug supply for most cancers therapy, however they nonetheless present some limitations equivalent to low photothermal conversion, poor photothermal stability, and improper temperature vary in biomedical functions.
  • On this paper, the two-dimensional MXene nanosheets with excessive photothermal conversion effectivity in addition to photothermal stability was firstly ready, then the MXene nanosheets and the therapeutic drug had been embedded within the low-melting-point agarose hydrogel community to manufacture the drug-loaded MXene/agarose hydrogel (MXene@Hydrogel). With the addition of low focus of MXene (20 ppm), the MXene@Hydrogel may rapidly rise to 60 °C below NIR irradiation and soften to launch the encapsulated medicine.
  • Importantly, the drug on/off launch and the kinetics may very well be simply managed with various agarose focus, MXene focus, gentle depth, and publicity time. As well as, the drug doxorubicin retained the anticancer exercise after launched from the MXene@Hydrogel community below NIR irradiation.
  • With the wonderful biocompatibility, the newly fabricated NIR-responsive MXene@Hydrogel affords a novel approach for the event of good hydrogel-based drug supply system for localized most cancers therapy.

 

FAM FLICA CASPASE-1 KIT (OKSA11275)

OKSA11275 100 Tests
EUR 1827.6
Description: Description of target: The FAM FLICATM Capase 1 kit uses a target sequence (YVAD) sandwiched between a green fluorescent label, FAM, and FMK to make a quick and flexible method to analyze active capases in apoptotic cells. These kits measure apoptosis by detecting active caspases in whole, living cells. They don't work by using antibodies or an ELISA. Instead, their methodology is based a unique cell-permeable and non-cytotoxic reagent called Fluorochrome Inhibitor of Caspases. These kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect, and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICA CASPASE-2 KIT (OKSA11283)

OKSA11283 100 Tests
EUR 1593.6
Description: Description of target: FAM FLICAâ„¢ CASPASE-2 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICA CASPASE-10 KIT (OKSA11285)

OKSA11285 100 Tests
EUR 1875.6
Description: Description of target: FAM FLICAâ„¢ CASPASE-10 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICA CASPASE-3/7 KIT (OKSA11271)

OKSA11271 100 Tests
EUR 1828.8
Description: Description of target: The FAM FLICA™ Caspase 3 & 7 kit uses a quick and easy method to analyze active caspases in apoptotic cells.;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

SR FLICAâ„¢ CASPASE-9 KIT (OKSA11301)

OKSA11301 25 Tests
EUR 724.8
Description: Description of target: SR FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

SR FLICAâ„¢ CASPASE-9 KIT (OKSA11302)

OKSA11302 100 Tests
EUR 1884
Description: Description of target: SR FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

Caspase 12 Assay Kit

55R-1290 25 assays
EUR 393.6
Description: Assay Kit for detection of Capase 12 activity in the research laboratory

Caspase 3 Assay Kit

20-abx294003
  • EUR 1506.00
  • EUR 548.40
  • EUR 924.00
  • 100 tests
  • 20 tests
  • 50 tests

Caspase 6 Assay Kit

20-abx299018
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Caspase 1 Assay Kit

20-abx299019
  • EUR 1638.00
  • EUR 754.80
  • 100 tests
  • 25 tests

Caspase 8 Assay Kit

20-abx299020
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Caspase 9 Assay Kit

20-abx299021
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Caspase 1 Assay Kit

abx299023-2550tests 25-50 tests
EUR 830.4

Caspase 2 Assay Kit

abx299026-25tests 25 tests
EUR 698.4

Caspase 10 Assay Kit

abx299027-100tests 100 tests
EUR 1562.4

Caspase 9 Assay Kit

20-abx299035
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Glycerol Cell-Based Assay Kit

K977-100
EUR 588

FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11268)

OKSA11268 25 Tests
EUR 709.2
Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11269)

OKSA11269 100 Tests
EUR 1828.8
Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

QuantiFluo Caspase-3 Assay Kit

DCS3-100 100
EUR 330
Description: Quantitative determination of the apoptosis target caspase-3 activity by fluorimetry (400/490nm) method. Procedure: 60 min. Kit size: 100 tests. Shelf life: 12 months.

Caspase 3 Fluorometric Assay Kit

55R-1269 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Caspase 3 Colorimetric Assay Kit

55R-1270 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Caspase 1 Fluorometric Assay Kit

55R-1272 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase 1 Colorimetric Assay Kit

55R-1273 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase 8 Fluorometric Assay Kit

55R-1274 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 8 activity in the research laboratory

Caspase 8 Colorimetric Assay Kit

55R-1275 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 8 activity in the research laboratory

Caspase 6 Fluorometric Assay Kit

55R-1276 25 assays
EUR 344.4
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 6 Colorimetric Assay Kit

55R-1277 25 assays
EUR 340.8
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 2 Fluorometric Assay Kit

55R-1278 25 assays
EUR 340.8
Description: Assay Kit for detection of Capase 2 activity in the research laboratory

Caspase 2 Colorimetric Assay Kit

55R-1279 25 assays
EUR 363.6
Description: Assay Kit for detection of Capase 2 activity in the research laboratory

Caspase 9 Fluorometric Assay Kit

55R-1280 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase 9 Colorimetric Assay Kit

55R-1281 25 assays
EUR 355.2
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase 5 Fluorometric Assay Kit

55R-1282 25 assays
EUR 340.8
Description: Assay Kit for detection of Capase 5 activity in the research laboratory

Caspase 5 Colorimetric Assay Kit

55R-1283 25 assays
EUR 363.6
Description: Assay Kit for detection of Capase 5 activity in the research laboratory

Caspase 10 Fluorometric Assay Kit

55R-1284 25 assays
EUR 340.8
Description: Assay Kit for detection of Capase 10 activity in the research laboratory

Caspase 10 Colorimetric Assay Kit

55R-1285 25 assays
EUR 363.6
Description: Assay Kit for detection of Capase 10 activity in the research laboratory

Caspase 4 Fluorometric Assay Kit

55R-1286 25 assays
EUR 363.6
Description: Assay Kit for detection of Capase 4 activity in the research laboratory

Caspase 4 Colorimetric Assay Kit

55R-1287 25 assays
EUR 374.4
Description: Assay Kit for detection of Capase 4 activity in the research laboratory

Caspase 3/7 Assay Kit

20-abx299017
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Caspase 3/7 Assay Kit

abx299024-2550tests 25-50 tests
EUR 830.4

Caspase 3/7 Assay Kit

20-abx299028
  • EUR 1431.60
  • EUR 698.40
  • 100 tests
  • 25 tests

Caspase-1 Activity Assay Kit

AR4003-100Assays 100 Assays
EUR 616.8

Caspase-1 Activity Assay Kit

AR4003-25Assays 25 Assays
EUR 283.2

Caspase-1 Activity Assay Kit

AR4003-50Assays 50 Assays
EUR 408

Caspase-2 Activity Assay Kit

AR4004-100Assays 100 Assays
EUR 616.8

Caspase-2 Activity Assay Kit

AR4004-25Assays 25 Assays
EUR 283.2

Caspase-2 Activity Assay Kit

AR4004-50Assays 50 Assays
EUR 408

Caspase 1 Assay Kit, green

KF17201 25 Tests
EUR 390

Caspase 2 Assay Kit, green

KF17202 25 Tests
EUR 364.8

Caspase 6 Assay Kit, green

KF17204 25 Tests
EUR 364.8

Caspase 8 Assay Kit, green

KF17205 25 Tests
EUR 364.8

Caspase 9 Assay Kit, green

KF17206 25 Tests
EUR 364.8

Caspase 10 Assay Kit, green

KF17207 25 Tests
EUR 364.8

Caspase 13 Assay Kit, green

KF17208 25 Tests
EUR 364.8

Caspase 9 Assay Kit, red

KF17302 25 Tests
EUR 364.8

660 Caspase-1 Assay Kit

KF17362 25-50 Tests
EUR 418.8

Caspase-5 Fluorometric Assay Kit

K2195-100 100 assays
EUR 602.4

Caspase-5 Fluorometric Assay Kit

K2195-200 200 assays
EUR 835.2

Caspase-5 Fluorometric Assay Kit

K2195-25 25 assays
EUR 285.6

Caspase-5 Fluorometric Assay Kit

K2195-400 400 assays
EUR 1336.8

Caspase-5 Colorimetric Assay Kit

K2196-100 100 assays
EUR 634.8

Caspase-5 Colorimetric Assay Kit

K2196-200 200 assays
EUR 870

Caspase-5 Colorimetric Assay Kit

K2196-25 25 assays
EUR 301.2

Caspase-5 Colorimetric Assay Kit

K2196-400 400 assays
EUR 1387.2

Caspase-10 Colorimetric Assay Kit

K2197-100 100 assays
EUR 634.8

Caspase-10 Colorimetric Assay Kit

K2197-200 200 assays
EUR 885.6

Caspase-10 Colorimetric Assay Kit

K2197-25 25 assays
EUR 301.2

Caspase-10 Colorimetric Assay Kit

K2197-400 400 assays
EUR 1336.8

Caspase-4 Fluorometric Assay Kit

K2198-100 100 assays
EUR 634.8

Caspase-4 Fluorometric Assay Kit

K2198-200 200 assays
EUR 885.6

Caspase-4 Fluorometric Assay Kit

K2198-25 25 assays
EUR 301.2

Caspase-4 Fluorometric Assay Kit

K2198-400 400 assays
EUR 1336.8

Caspase-4 Colorimetric Assay Kit

K2199-100 100 assays
EUR 668.4

Caspase-4 Colorimetric Assay Kit

K2199-200 200 assays
EUR 885.6

Caspase-4 Colorimetric Assay Kit

K2199-25 25 assays
EUR 319.2

Caspase-4 Colorimetric Assay Kit

K2199-400 400 assays
EUR 1336.8

Caspase-12 Fluorometric Assay Kit

K139-100
EUR 601.2

Caspase-12 Fluorometric Assay Kit

K139-25
EUR 307.2

Caspase-6 Fluorometric Assay Kit

K114-100
EUR 516

Caspase-6 Fluorometric Assay Kit

K114-200
EUR 738

Caspase-6 Fluorometric Assay Kit

K114-25
EUR 242.4

Caspase-6 Fluorometric Assay Kit

K114-400
EUR 1149.6

Caspase-6 Colorimetric Assay Kit

K115-100
EUR 542.4

Caspase-6 Colorimetric Assay Kit

K115-200
EUR 738

Caspase-6 Colorimetric Assay Kit

K115-25
EUR 242.4

Caspase-6 Colorimetric Assay Kit

K115-400
EUR 1149.6

Caspase-2 Fluorometric Assay Kit

K116-100
EUR 555.6

Caspase-2 Fluorometric Assay Kit

K116-200
EUR 738

Caspase-2 Fluorometric Assay Kit

K116-25
EUR 242.4

Caspase-2 Fluorometric Assay Kit

K116-400
EUR 1149.6

Caspase-2 Colorimetric Assay Kit

K117-100
EUR 561.6

Caspase-2 Colorimetric Assay Kit

K117-200
EUR 738

Caspase-2 Colorimetric Assay Kit

K117-25
EUR 255.6

Caspase-2 Colorimetric Assay Kit

K117-400
EUR 1149.6

Caspase-9 Fluorometric Assay Kit

K118-100
EUR 561.6