2 Apb 4-Hne Antibody Anti-Ubiquitin Antibody Arf1 Antibody azide azide free Cytokine Array Mouse Ddr2 Antibody E coli Elisa Array Human Kd Antibody Kdel Antibody Lizard Llama Max Antibody Mettl3 Antibody Mink Mouse Opossum Orangutan Tyrosinase Antibody What Are Feeder Cells
Photothermal Agarose Microfabrication Technology for Collective Cell Migration Analysis
Agarose photothermal microfabrication know-how is among the micropatterning methods that has the benefit of easy and versatile real-time fabrication even in the course of the cultivation of cells. To look at the flexibility and limitation of the agarose microstructures, we investigated the collective epithelial cell migration habits in two-dimensional agarose confined buildings. Agarose microchannels from 10 to 211 micrometer width had been fabricated with a spot heating of a centered 1480 nm wavelength infrared laser to the skinny agarose layer coated on the cultivation dish after the cells occupied the reservoir.
The collective cell migration velocity maintained fixed no matter their extension distance, whereas the width dependency of these velocities was maximized round 30 micrometer width and decreased each within the narrower and wider microchannels. The only-cell monitoring revealed that the lower of velocity within the narrower width was brought on by the obvious improve of facet ratio of cell form (as much as 8.9). In distinction, the lower within the wider channels was primarily brought on by the rise of the random walk-like habits of part cells. The outcomes confirmed the benefits of this technique: (1) versatile fabrication with none pre-designing, (2) modification even throughout cultivation, and (3) the cells had been confined within the agarose geometry.
Semi-Computerized Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Purposes
On this paper, a prototype of a semi-automatic lab-on-PCB for agarose gel preparation and electrophoresis is developed. The scale of the machine are 38 × 34 mm2 and it features a conductivity sensor for detecting the TAE buffer (Tris-acetate-EDTA buffer), a microheater for growing the solubility of the agarose, a unfavorable temperature coefficient (NTC) thermistor for controlling the temperature, a lightweight dependent resistor (LDR) sensor for measuring the transparency of the combination, and two electrodes for performing the electrophoresis. The agarose preparation features are ruled by a microcontroller.
The machine requires a PMMA construction to outline the wells of the agarose gel, and to launch the electrodes from the agarose. The utmost voltage and present that the system requires are 40 V to carry out the electrophoresis, and 1 A for activating the microheater. The chosen temperature for mixing is 80 ∘C, with a mixing time of 10 min. As well as, the curing time is about 30 min. This machine is meant to be built-in as part of a bigger lab-on-PCB system for DNA amplification and detection. Nonetheless, it may be used emigrate DNA amplified in standard thermocyclers. Furthermore, the machine might be modified for getting ready bigger agarose gels and performing electrophoresis.
Novel Thermotolerant Amylase from Bacillus licheniformis Pressure LB04: Purification, Characterization and Agar-Agarose
This examine analyzed the thermostability and impact of calcium ions on the enzymatic exercise of α-amylase produced by Bacillus licheniformis pressure LB04 remoted from Espinazo Scorching springs in Nuevo Leon, Mexico. The enzyme was immobilized by entrapment on agar-agarose beads, with an entrapment yield of 19.9%. The identification of the micro organism was carried out utilizing 16s rDNA sequencing.
The enzyme was purified by way of ion trade chromatography (IEX) in a DEAE-Sephadex column, revealing a protein with a molecular weight of ≈130 kDa. The enzyme was steady at pH 3.Zero and warmth steady as much as 80 °C. Nonetheless, the optimum circumstances had been reached at 65 °C and pH 3.0, with a selected exercise of 1851.7 U mg-1 ± 1.3. The agar-agarose immobilized α-amylase had a hydrolytic exercise almost 25% larger when in comparison with the free enzyme. This examine gives important data for the understanding of the enzymatic profile of B. licheniformis pressure LB04 and the potential utility of the microorganisms at an industrial stage, particularly within the meals business.
Chemotaxis: Underneath Agarose Assay
The unicellular eukaryotic amoeba, Dictyostelium discoideum, represents an excellent mannequin for inspecting the molecular mechanism of chemotaxis. Underneath vegetative circumstances, the amoebae are chemotactically conscious of pterins, equivalent to folic acid. Underneath starved circumstances, they lose their sensitivity to pterins and turn out to be chemotactically conscious of cAMP. As an NIH mannequin system, Dictyostelium affords quite a lot of benefits in learning chemotaxis, together with ease of development, genetic tractability, and the conservation of mammalian signaling pathways. On this chapter, we describe the usage of the under-agarose chemotaxis assay to grasp the signaling pathways controlling directional sensing and motility in Dictyostelium discoideum. Given the similarities between Dictyostelium and mammalian cells, this permits us to dissect conserved pathways concerned in eukaryotic chemotaxis.
Expression and Ni-NTA-Agarose Purification of Recombinant Hepatitis C Virus E2Ectodomain Produced in a Baculovirus Expression System
On this protocol, we describe the manufacturing and purification of the ectodomain of the E2661 envelope protein (amino acids 384-661) of the Hepatitis C virus, which performs a elementary function within the entry of the virus into the host cell. This protein has been expressed in each prokaryotic and eukaryotic programs however in small portions or with out native protein traits.
- In our case, we use the Baculovirus expression system in insect cells. E2661 is secreted into the extracellular medium and purified via affinity chromatography a Ni-NTA-column as a result of the protein has a tag of six histidines at its amino terminal finish.
- The purified protein possesses a native-like conformation and it’s produced in giant portions, round 5-6 mg per liter.
- Sensible hydrogels conscious of minimally invasive near-infrared (NIR) gentle have nice potential in localized drug supply for most cancers therapy, however they nonetheless present some limitations equivalent to low photothermal conversion, poor photothermal stability, and improper temperature vary in biomedical functions.
- On this paper, the two-dimensional MXene nanosheets with excessive photothermal conversion effectivity in addition to photothermal stability was firstly ready, then the MXene nanosheets and the therapeutic drug had been embedded within the low-melting-point agarose hydrogel community to manufacture the drug-loaded MXene/agarose hydrogel (MXene@Hydrogel). With the addition of low focus of MXene (20 ppm), the MXene@Hydrogel may rapidly rise to 60 °C below NIR irradiation and soften to launch the encapsulated medicine.
- Importantly, the drug on/off launch and the kinetics may very well be simply managed with various agarose focus, MXene focus, gentle depth, and publicity time. As well as, the drug doxorubicin retained the anticancer exercise after launched from the MXene@Hydrogel community below NIR irradiation.
- With the wonderful biocompatibility, the newly fabricated NIR-responsive MXene@Hydrogel affords a novel approach for the event of good hydrogel-based drug supply system for localized most cancers therapy.
) FAM FLICA CASPASE-1 KIT (OKSA11275) |
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| OKSA11275 | Aviva Systems Biology | 100 Tests | EUR 1523 |
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Description: Description of target: The FAM FLICATM Capase 1 kit uses a target sequence (YVAD) sandwiched between a green fluorescent label, FAM, and FMK to make a quick and flexible method to analyze active capases in apoptotic cells. These kits measure apoptosis by detecting active caspases in whole, living cells. They don't work by using antibodies or an ELISA. Instead, their methodology is based a unique cell-permeable and non-cytotoxic reagent called Fluorochrome Inhibitor of Caspases. These kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect, and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity: |
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) FAM FLICA CASPASE-2 KIT (OKSA11283) |
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| OKSA11283 | Aviva Systems Biology | 100 Tests | EUR 1328 |
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Description: Description of target: FAM FLICAâ„¢ CASPASE-2 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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) FAM FLICA CASPASE-10 KIT (OKSA11285) |
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| OKSA11285 | Aviva Systems Biology | 100 Tests | EUR 1563 |
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Description: Description of target: FAM FLICAâ„¢ CASPASE-10 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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) FAM FLICA CASPASE-3/7 KIT (OKSA11271) |
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| OKSA11271 | Aviva Systems Biology | 100 Tests | EUR 1524 |
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Description: Description of target: The FAM FLICA™ Caspase 3 & 7 kit uses a quick and easy method to analyze active caspases in apoptotic cells.;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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) SR FLICAâ„¢ CASPASE-9 KIT (OKSA11301) |
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| OKSA11301 | Aviva Systems Biology | 25 Tests | EUR 604 |
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Description: Description of target: SR FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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) SR FLICAâ„¢ CASPASE-9 KIT (OKSA11302) |
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| OKSA11302 | Aviva Systems Biology | 100 Tests | EUR 1570 |
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Description: Description of target: SR FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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 Caspase 3 Assay Kit |
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| 20-abx294003 | Abbexa |
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 Caspase 6 Assay Kit |
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| 20-abx299018 | Abbexa |
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 Caspase 1 Assay Kit |
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| 20-abx299019 | Abbexa |
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 Caspase 8 Assay Kit |
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| 20-abx299020 | Abbexa |
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 Caspase 9 Assay Kit |
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| 20-abx299021 | Abbexa |
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Caspase 1 Assay Kit |
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| abx299023-2550tests | Abbexa | 25-50 tests | EUR 692 |
 Caspase 2 Assay Kit |
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| abx299026-25tests | Abbexa | 25 tests | EUR 582 |
 Caspase 10 Assay Kit |
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| abx299027-100tests | Abbexa | 100 tests | EUR 1302 |
Caspase 9 Assay Kit |
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| 20-abx299035 | Abbexa |
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 Caspase 12 Assay Kit |
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| 55R-1290 | Fitzgerald | 25 assays | EUR 328 |
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Description: Assay Kit for detection of Capase 12 activity in the research laboratory |
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 Glycerol Cell-Based Assay Kit |
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| K977-100 | Biovision | EUR 490 | |
) FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11268) |
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| OKSA11268 | Aviva Systems Biology | 25 Tests | EUR 591 |
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Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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) FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11269) |
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| OKSA11269 | Aviva Systems Biology | 100 Tests | EUR 1524 |
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Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity: |
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 Caspase 1 Assay Kit, green |
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| KF17201 | Neuromics | 25 Tests | EUR 325 |
 Caspase 2 Assay Kit, green |
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| KF17202 | Neuromics | 25 Tests | EUR 304 |
 Caspase 6 Assay Kit, green |
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| KF17204 | Neuromics | 25 Tests | EUR 304 |
 Caspase 8 Assay Kit, green |
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| KF17205 | Neuromics | 25 Tests | EUR 304 |
 Caspase 9 Assay Kit, green |
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| KF17206 | Neuromics | 25 Tests | EUR 304 |
 Caspase 10 Assay Kit, green |
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| KF17207 | Neuromics | 25 Tests | EUR 304 |
 Caspase 13 Assay Kit, green |
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| KF17208 | Neuromics | 25 Tests | EUR 304 |
 Caspase 9 Assay Kit, red |
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| KF17302 | Neuromics | 25 Tests | EUR 304 |
 660 Caspase-1 Assay Kit |
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| KF17362 | Neuromics | 25-50 Tests | EUR 349 |
 Caspase-1 Activity Assay Kit |
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| AR4003-100Assays | BosterBio | 100 Assays | EUR 514 |
Caspase-1 Activity Assay Kit |
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| AR4003-25Assays | BosterBio | 25 Assays | EUR 236 |
Caspase-1 Activity Assay Kit |
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| AR4003-50Assays | BosterBio | 50 Assays | EUR 340 |
 Caspase-2 Activity Assay Kit |
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| AR4004-100Assays | BosterBio | 100 Assays | EUR 514 |
Caspase-2 Activity Assay Kit |
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| AR4004-25Assays | BosterBio | 25 Assays | EUR 236 |
Caspase-2 Activity Assay Kit |
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| AR4004-50Assays | BosterBio | 50 Assays | EUR 340 |
 Caspase 3/7 Assay Kit |
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| 20-abx299017 | Abbexa |
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Caspase 3/7 Assay Kit |
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| abx299024-2550tests | Abbexa | 25-50 tests | EUR 692 |
Caspase 3/7 Assay Kit |
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| 20-abx299028 | Abbexa |
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 Caspase-3 Fluorometric Assay Kit |
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| K2007-200 | ApexBio | 200 assays | EUR 696 |
Caspase-3 Fluorometric Assay Kit |
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| K2007-25 | ApexBio | 25 assays | EUR 238 |
Caspase-3 Fluorometric Assay Kit |
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| K2007-400 | ApexBio | 400 assays | EUR 1086 |
 Caspase-3 Colorimetric Assay Kit |
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| K2008-200 | ApexBio | 200 assays | EUR 696 |
Caspase-3 Colorimetric Assay Kit |
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| K2008-25 | ApexBio | 25 assays | EUR 238 |
Caspase-3 Colorimetric Assay Kit |
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| K2008-400 | ApexBio | 400 assays | EUR 1086 |
 Caspase-1 Fluorometric Assay Kit |
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| K2010-200 | ApexBio | 200 assays | EUR 696 |
Caspase-1 Fluorometric Assay Kit |
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| K2010-25 | ApexBio | 25 assays | EUR 238 |
Caspase-1 Fluorometric Assay Kit |
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| K2010-400 | ApexBio | 400 assays | EUR 1086 |
 Caspase-1 Colorimetric Assay Kit |
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| K2011-200 | ApexBio | 200 assays | EUR 696 |
Caspase-1 Colorimetric Assay Kit |
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| K2011-25 | ApexBio | 25 assays | EUR 238 |
Caspase-1 Colorimetric Assay Kit |
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| K2011-400 | ApexBio | 400 assays | EUR 1086 |
 Caspase-8 Fluorometric Assay Kit |
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| K2012-200 | ApexBio | 200 assays | EUR 696 |
Caspase-8 Fluorometric Assay Kit |
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| K2012-25 | ApexBio | 25 assays | EUR 238 |
Caspase-8 Fluorometric Assay Kit |
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| K2012-400 | ApexBio | 400 assays | EUR 1086 |
 Caspase-8 Colorimetric Assay Kit |
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| K2013-200 | ApexBio | 200 assays | EUR 696 |
Caspase-8 Colorimetric Assay Kit |
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| K2013-25 | ApexBio | 25 assays | EUR 238 |
Caspase-8 Colorimetric Assay Kit |
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| K2013-400 | ApexBio | 400 assays | EUR 1086 |
 Caspase-6 Fluorometric Assay Kit |
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| K2014-200 | ApexBio | 200 assays | EUR 696 |
Caspase-6 Fluorometric Assay Kit |
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| K2014-25 | ApexBio | 25 assays | EUR 238 |
Caspase-6 Fluorometric Assay Kit |
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| K2014-400 | ApexBio | 400 assays | EUR 1086 |
 Caspase-6 Colorimetric Assay Kit |
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| K2015-200 | ApexBio | 200 assays | EUR 696 |
Caspase-6 Colorimetric Assay Kit |
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| K2015-25 | ApexBio | 25 assays | EUR 238 |
Caspase-6 Colorimetric Assay Kit |
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| K2015-400 | ApexBio | 400 assays | EUR 1101 |
 Caspase-2 Fluorometric Assay Kit |
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| K2016-200 | ApexBio | 200 assays | EUR 696 |
Caspase-2 Fluorometric Assay Kit |
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| K2016-25 | ApexBio | 25 assays | EUR 238 |
Caspase-2 Fluorometric Assay Kit |
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| K2016-400 | ApexBio | 400 assays | EUR 1114 |
 Caspase-2 Colorimetric Assay Kit |
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| K2017-200 | ApexBio | 200 assays | EUR 725 |
Caspase-2 Colorimetric Assay Kit |
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| K2017-25 | ApexBio | 25 assays | EUR 251 |
Caspase-2 Colorimetric Assay Kit |
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| K2017-400 | ApexBio | 400 assays | EUR 1156 |
 Caspase-9 Fluorometric Assay Kit |
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| K2018-200 | ApexBio | 200 assays | EUR 669 |
Caspase-9 Fluorometric Assay Kit |
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| K2018-25 | ApexBio | 25 assays | EUR 238 |
Caspase-9 Fluorometric Assay Kit |
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| K2018-400 | ApexBio | 400 assays | EUR 1114 |
 Caspase-9 Colorimetric Assay Kit |
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| K2019-200 | ApexBio | 200 assays | EUR 696 |
Caspase-9 Colorimetric Assay Kit |
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| K2019-25 | ApexBio | 25 assays | EUR 251 |
Caspase-9 Colorimetric Assay Kit |
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| K2019-400 | ApexBio | 400 assays | EUR 1156 |
 Caspase-12 Fluorometric Assay Kit |
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| K2150-100 | ApexBio | 100 assays | EUR 529 |
Caspase-12 Fluorometric Assay Kit |
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| K2150-25 | ApexBio | 25 assays | EUR 251 |
 Caspase-5 Fluorometric Assay Kit |
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| K2195-100 | ApexBio | 100 assays | EUR 502 |
Caspase-5 Fluorometric Assay Kit |
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| K2195-200 | ApexBio | 200 assays | EUR 696 |
Caspase-5 Fluorometric Assay Kit |
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| K2195-25 | ApexBio | 25 assays | EUR 238 |
Caspase-5 Fluorometric Assay Kit |
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| K2195-400 | ApexBio | 400 assays | EUR 1114 |
 Caspase-5 Colorimetric Assay Kit |
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| K2196-100 | ApexBio | 100 assays | EUR 529 |
Caspase-5 Colorimetric Assay Kit |
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| K2196-200 | ApexBio | 200 assays | EUR 725 |
Caspase-5 Colorimetric Assay Kit |
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| K2196-25 | ApexBio | 25 assays | EUR 251 |
Caspase-5 Colorimetric Assay Kit |
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| K2196-400 | ApexBio | 400 assays | EUR 1156 |
 Caspase-10 Colorimetric Assay Kit |
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| K2197-100 | ApexBio | 100 assays | EUR 529 |
Caspase-10 Colorimetric Assay Kit |
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| K2197-200 | ApexBio | 200 assays | EUR 738 |
Caspase-10 Colorimetric Assay Kit |
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| K2197-25 | ApexBio | 25 assays | EUR 251 |
Caspase-10 Colorimetric Assay Kit |
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| K2197-400 | ApexBio | 400 assays | EUR 1114 |
 Caspase-4 Fluorometric Assay Kit |
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| K2198-100 | ApexBio | 100 assays | EUR 529 |
Caspase-4 Fluorometric Assay Kit |
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| K2198-200 | ApexBio | 200 assays | EUR 738 |
Caspase-4 Fluorometric Assay Kit |
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| K2198-25 | ApexBio | 25 assays | EUR 251 |
Caspase-4 Fluorometric Assay Kit |
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| K2198-400 | ApexBio | 400 assays | EUR 1114 |
 Caspase-4 Colorimetric Assay Kit |
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| K2199-100 | ApexBio | 100 assays | EUR 557 |
Caspase-4 Colorimetric Assay Kit |
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| K2199-200 | ApexBio | 200 assays | EUR 738 |
Caspase-4 Colorimetric Assay Kit |
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| K2199-25 | ApexBio | 25 assays | EUR 266 |
Caspase-4 Colorimetric Assay Kit |
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| K2199-400 | ApexBio | 400 assays | EUR 1114 |
