BT3 Technologies

Cellufine, agarose, BT10, Barrier tips, agar, His-tag

bt3technologies
2 Apb 4-Hne Antibody Arf1 Antibody azide azide free Cytokine Array Mouse Donkey E coli Elisa Array Influenza Kd Antibody Kdel Antibody Llama Mettl3 Antibody Mink Mouse Orangutan What Are Feeder Cells

Fabrication of Flexible pH-Responsive Agarose/Succinoglycan Hydrogels for Controlled Drug Release

Agarose/succinoglycan hydrogels have been ready as pH-responsive drug supply techniques with considerably improved flexibility, thermostability, and porosity in comparison with agarose gels alone. Agarose/succinoglycan hydrogels have been made utilizing agarose and succinoglycan, a polysaccharide straight remoted from Sinorhizobium meliloti. Mechanical and bodily properties of agarose/succinoglycan hydrogels have been investigated utilizing varied instrumental strategies similar to rheological measurements, attenuated whole reflection-Fourier rework infrared (ATR-FTIR) spectroscopic evaluation, X-ray diffraction (XRD), and field-emission scanning electron microscopy (FE-SEM).
The outcomes confirmed that the agarose/succinoglycan hydrogels turned versatile and steady community gels with an improved swelling sample in primary answer in comparison with the arduous and brittle agarose gel alone. As well as, these hydrogels confirmed a pH-responsive supply of ciprofloxacin (CPFX), with a cumulative launch of ~41% inside 35 h at pH 1.2 and full launch at pH 7.4. Agarose/succinoglycan hydrogels additionally proved to be non-toxic because of the cell cytotoxicity take a look at, suggesting that these hydrogels could be a possible pure biomaterial for biomedical functions similar to varied drug supply system and cell tradition scaffolds.

1.eight V Aqueous Symmetric Carbon-Based mostly Supercapacitors with Agarose-Certain Activated Carbons in an Acidic Electrolyte

 

The precise vitality of an aqueous carbon supercapacitor is mostly small, ensuing primarily from a slender potential window of aqueous electrolytes. Right here, we launched agarose, an ecologically appropriate polymer, as a novel binder to manufacture an activated carbon supercapacitor, enabling a wider potential window attributed to a excessive overpotential of the hydrogen-evolution response (HER) of agarose-bound activated carbons in sulfuric acid.
Assembled symmetric aqueous cells could be galvanostatically cycled as much as 1.eight V, attaining an enhanced vitality density of 13.5 W h/kg (9.5 µW h/cm2) at 450 W/kg (315 µW/cm2). Moreover, an amazing biking habits was obtained, with a 94.2% retention of capacitance after 10,000 cycles at 2 A/g. This work would possibly information the design of another materials for high-energy aqueous supercapacitors.
Sort 1 diabetic sufferers with extreme hypoglycemia unawareness have benefitted from mobile therapies, similar to pancreas or islet transplantation; nevertheless, donor scarcity and the necessity for immunosuppression limits widespread medical software. We beforehand developed an intravascular bioartificial pancreas (iBAP) utilizing silicon nanopore membranes (SNM) for immunoprotection.
To make sure ample nutrient supply, the iBAP will want a cell scaffold with excessive hydraulic permeability to supply mechanical assist and preserve islet viability and performance. Right here, we study the feasibility of superporous agarose (SPA) as a possible cell scaffold within the iBAP. SPA reveals 66-fold larger hydraulic permeability than the SNM together with a brief (<10 μm) diffusion distance to the closest islet.
SPA additionally helps short-term performance of each encapsulated human islets and stem-cell-derived enriched β-clusters in a convection-based system, demonstrated by excessive viability (>95%) and biphasic insulin responses to dynamic glucose stimulus. These findings recommend that the SPA scaffold won’t restrict nutrient supply in a convection-based bioartificial pancreas and deserves continued investigation.

Hepatotoxicity of copper sulfide nanoparticles in direction of hepatocyte spheroids utilizing a novel multi-concave agarose chip methodology

Goal: To discover the hepatotoxicity of copper sulfide nanoparticles (CuSNPs) towards hepatocyte spheroids.
Supplies & strategies: Aside from the normal agarose methodology to generate hepatocyte spheroids, we developed a multi-concave agarose chip (MCAC) methodology to research adjustments in hepatocyte viability, morphology, mitochondrial membrane potential, reactive oxygen species and hepatobiliary transporter by CuSNPs.
Outcomes: The MCAC methodology allowed a lot of spheroids to be obtained per pattern. CuSNPs confirmed hepatotoxicity in vitro via a lower in spheroid viability, albumin/urea manufacturing and glycogen deposition. CuSNPs additionally launched hepatocyte spheroid damage via alteration of mitochondrial membrane potential and reactive oxygen species, that might be reversed by N-acetyl-l-cysteine. CuSNPs considerably decreased the exercise of BSEP transporter by downregulating its mRNA and protein ranges. Exercise of the MRP2 transporter remained unchanged.
 Conclusion: We noticed the hepatotoxicity of CuSNPs in vitro with related mechanisms in a sophisticated 3D tradition system.

A Novel Technique to Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based mostly Hydrogels for the Managed Launch of Gentamicin

Introduction: Using spinal implants for the remedy of again issues is basically affected by the insurgence of infections on the implantation web site. Antibacterial coatings have been proposed as a viable answer to restrict such infections. Nevertheless, regardless of being efficient at short-term, standard coatings lack the power to forestall infections at medium and long-term. Hydrogel-based drug supply techniques could symbolize an answer controlling the discharge of the loaded antibacterial brokers whereas bettering cell integration. Agarose, specifically, is a biocompatible pure polysaccharide identified to enhance cell development and already utilized in drug supply system formulations. On this examine, an agarose hydrogel-based coating has been developed for the managed launch of gentamicin (GS).
Strategies: Sand blasted Ti6Al4V discs have been grafted with dopamine (DOPA) answer. After, GS-loaded agarose hydrogels have been produced and additioned with tannic acid (TA) and calcium chloride (CaCl2) as crosslinkers. The totally different GS-loaded hydrogel formulations have been deposited on Ti6Al4V-DOPA surfaces, and allowed to react below UV irradiation. Floor topography, wettability and composition have been analyzed with profilometry, static contact angle measurement, XPS and FTIR spectroscopy analyses. GS launch was carried out below pseudo-physiological circumstances as much as 28 days and the launched GS was quantified utilizing a selected ELISA take a look at. The cytotoxicity of the produced coatings towards human cells have been examined, together with their antibacterial exercise towards S. aureus micro organism.
Outcomes: A homogeneous coating was obtained with all of the hydrogel formulations. Furthermore, the coatings introduced a hydrophilic habits and micro-scale floor roughness. The addition of TA within the hydrogel formulations confirmed a rise within the launch time in comparison with the traditional GS-agarose hydrogels. Furthermore, the GS launched from these gels was capable of considerably inhibit S. aureus development in comparison with the GS-agarose hydrogels. The addition of CaCl2 to the gel formulation was capable of considerably lower cytotoxicity of the TA-modified hydrogels.
Conclusions: As a consequence of their floor properties, low cytotoxicity and excessive antibacterial results, the hereby proposed gentamicin-loaded agarose-hydrogels present new perception, and symbolize a promising method for the floor modification of spinal implants, enormously impacting their software within the orthopedic surgical situation.
bt3technologies
bt3technologies

Enhancement of emulsion penetration in agarose gel mannequin utilizing versatile plasma remedy

Transdermal supply via the pores and skin is a gorgeous various to injection as a result of it’s potential to launch a drug with much less ache. We ready a versatile plasma patch (FPP) utilizing printed electronics methods and examined transdermal supply enhancement of a water emulsion in agarose gel in anin vitrotissue mannequin.
At room temperature, the FPP generated chilly air plasma, which provided reactive oxygen species similar to ozone on the floor of the agarose gel. The FPP remedy was efficient in enhancing the water emulsion transport into the agarose gel. The penetration depth of the emulsion within the non-plasma-treated 3% agarose gel mannequin was roughly 0.69 mm whereas that of the plasma-treated mannequin was roughly 1.21 mm.

Blocking Buffer for ELISA
abx090807-500ml 500 ml
EUR 384

Neptune Assay Diluent-AD3
KF17349 500 ml
EUR 244

Neptune Sample Diluent-SD3
KF17354 500 ml
EUR 244

Neptune 10ul barrier tip
BT10 10 trays of 96 / pack
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 100ul barrier tip
BT100 10 trays of 96 / pack
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 1000ul barrier tip
BT1000.96 8 trays of 96 / pack
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 20ul barrier tip
BT20 10 trays of 96 / pack
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 200ul barrier tip
BT200 10 trays of 96 / pack
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 300ul barrier tip
BT300 10 trays of 96 / pack
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Blocking Buffer, 10X, 250ML
X109-250ML 250ML
EUR 359

Blocking Buffer, 10X, 25ML
X109-25ML 25ML
EUR 122

General Blocking Buffer-BB1
KF17337 500 ml
EUR 231

SynBlock Blocking Buffer-BB3
KF17339 500 ml
EUR 244

TBS Blocking Buffer Pack
AR0143 200mL/pack
EUR 65

EZBlock? (TBS) Blocking Buffer
2117-1000
EUR 212

EZBlock? (TBS) Blocking Buffer
2117-200
EUR 125

EZBlock? (PBS) Blocking Buffer
2128-1000
EUR 229

EZBlock? (PBS) Blocking Buffer
2128-200
EUR 137

Block
H5000-02 1 PC
EUR 290.33

Block
H5000-025 1 PC
EUR 591.35

Block
H5000-05 1 PC
EUR 290.33

Block
H5000-12 1 PC
EUR 382.55

Block
H5000-1232 1 PC
EUR 382.55

Block
H5000-15 1 PC
EUR 290.33

Block
H5000-150 1 PC
EUR 382.55

Block
H5000-20 1 PC
EUR 382.55

Block
H5000-500 1 PC
EUR 382.55

Block
H5000-CMB 1 PC
EUR 290.33

Block
H5000-CU 1 PC
EUR 382.55

Block
H5000-WB 1 PC
EUR 4288.85

Block
BSH100-02 1 PC
EUR 137.21

Block
BSH100-05 1 PC
EUR 146.78

Block
BSH100-1213 1 PC
EUR 137.21

Block
BSH100-1232 1 PC
EUR 137.21

Block
BSH100-15 1 PC
EUR 137.21

Block
BSH100-150 1 PC
EUR 156.35

Block
BSH100-1520 1 PC
EUR 137.21

Block
BSH100-15HL 1 PC
EUR 156.35

Block
BSH100-500 1 PC
EUR 104.15

Block
BSH100-5MT 1 PC
EUR 156.35

Block
BSH100-CV 1 PC
EUR 137.21

Block
BSW02 1 PC
EUR 151.93

Block
BSW05 1 PC
EUR 151.93

Block
BSW10 1 PC
EUR 151.93

Block
BSW13 1 PC
EUR 151.93

Block
BSW15 1 PC
EUR 151.93

Block
BSW1500 1 PC
EUR 151.93

Block
BSW1516 1 PC
EUR 183.03

Block
BSW1520 1 PC
EUR 151.93

Block
BSW50 1 PC
EUR 151.93

Block
BSW5MT 1 PC
EUR 151.93

Block
BSWHEM 1 PC
EUR 237.26

Block
BSWMT 1 PC
EUR 194.99

Block
BSWNMR 1 PC
EUR 481.73

Block
BSWPCR1 1 PC
EUR 255.6

Block
BSWPCR2 1 PC
EUR 208.55

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed
ELISA-1 1
EUR 202

Neptune 1000-1250ul barrier tip
BT1250 8 trays of 96 / pack
EUR 59
Description: Extended Length, Low retention, Racked, Pre-Sterile, Sample Saving Surface

TBS blocking buffer with BSA
SD8113 1pk, 1L
EUR 83.93

PBS Blocking buffer with BSA
SB0626 1pk, 1L
EUR 83.93

3 Blocking Buffer Sample Pack
KF17340 3 x 100 ml
EUR 205

Permeabilization and Blocking Buffer (5X)
22017 100mL
EUR 149
Description: Minimum order quantity: 1 unit of 100mL

TrueBlack WB Blocking Buffer Kit
23013 1kit
EUR 347
Description: Minimum order quantity: 1 unit of 1kit

TrueBlack WB Blocking Buffer Kit
23013-T 1kit
EUR 125
Description: Minimum order quantity: 1 unit of 1kit

Blocking Buffer and Coating Stabilizer
abx090811-1000ml 1000 ml
EUR 523

West-Ezier Super Blocking Buffer
W3700-010 100ml
EUR 97

West-Ezier Super Blocking Buffer
W3700-050 500ml
EUR 165

West-Ezier Super Blocking Buffer
W3700-100 1L
EUR 246

10 x Universal Blocking Buffer
GR103005 200 mL
EUR 129

EZBlock? T20 (TBS) Blocking Buffer
2140-1000
EUR 218

EZBlock? T20 (TBS) Blocking Buffer
2140-200
EUR 120

EZBlock? T20 (PBS) Blocking Buffer
2143-1000
EUR 218

EZBlock? T20 (PBS) Blocking Buffer
2143-200
EUR 120

EZ Block
EZB125 125 ml
EUR 93

EZ Block
EZB500 500 ml
EUR 127

EZ Block
EZB999 1000 ml
EUR 173

Pro-Block
PBK-20000 20 L
EUR 1210

Pro-Block
PBK010 10 L
EUR 676

Pro-Block
PBK125 125 ml
EUR 74

Pro-Block
PBK500 500 ml
EUR 101

Pro-Block
PBK999 1000 ml
EUR 124

Super Block
AAA125 125 ml
EUR 74

Super Block
AAA500 500 ml
EUR 101

Super Block
AAA999 1000 ml
EUR 124

Peroxide Block
ACA015 15 ml
EUR 67

Peroxide Block
ACA125 125 ea.
EUR 76

Peroxide Block
ACA500 500 ml
EUR 101

Peroxide Block
ACA999 1000 ml
EUR 125

Block-Free ELISA Reagent
M4102-250
EUR 452

Block-Free ELISA Reagent
M4102-50
EUR 147

Neptune 10ul extended length barrier tip
BT10XLS3 10 trays of 96 / pack
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface
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bt3technologies
bt3technologies