BT3 Technologies

6 Is your exosome prep contaminated with IgG and/or beads?
A: We use explicit reagents to grab exosomes. We did not use antibody or beads for the isolation. So the final word exosome prep is simply not contaminated with artificial IgG or beads.

7, Do your exosome prep accommodates Albumin?
A: Some sorts of cells paying homage to hepatocytes carries enriched Albumin, and these liver derived exosomes will probably be launched into the circulation system. So, if the exosomes from these types of samples are isolated, it’d accommodates Albumin.

8, Can I benefit from your PureExo gear (Cat. #P100) on our D.discoideum amoeba?
A: PureExo exosome isolation gear captures launched exosomes or microvesicles from cells. For amoeba exosome isolation, we advise to collect amoeba custom medium because the an identical approach to collect cell custom medium.

9, What is the composition of the buffer that the final word exosomes prep may be in? Will it have an effect on my downstream assay?
A: The last word isolated exosomes are aggregated nano-particles mixed with a very small amount of genuine custom medium or serum. PureExo Exosome Isolation gear provides intact exosomes which allows for a sorts of downstream functions along with exosome flooring labeling10， How have you ever learnt that an exosome prep is pure, free of completely different membrane-derived microparticles
(shredding vesicles)?
A: The isolated microvesicle from cell custom medium or serum using our isolation kits confirmed sphere membrane encapsulated particles with the diameters numerous between 20-200nm beneath EM scanning. These characters help to seek out out that the harvested microvesicle are exosomes. Shedding particles are reported to be irregular type with diameter as a lot as 1000nm. In addition to, the dimensions distribution pattern is confirmed by dynamic gentle scattering indicating the isolated microvesicles are of diameter between 100 and 250nm.

11， Are exosome functionally energetic?
A: Our collaborators used our kits to detect explicit mirRNAs carried by exosomes. Luciferase train assay
confirmed energetic carry out of exosomal microRNA after the exosomes have been utilized to cells transfected with specializing in mRNA 3’UTR vector.

12， Is it essential to utilize glass tubes?
A: Using glass tubes instead of plastic tubes is required in step Three and step 4.
Please uncover glass tubes from FisherScientific. http://www.fishersci.com (Look for Fisherbrand* Disposable
Borosilicate Glass Tubes Cat. NO: 14-961-26).

13， Why can’t I see clear fluff heart layer?
A: The following coping with errors may end result within the failure:
1). Reply A/B/C did not mix completely sooner than together with the cell custom medium or serum.
2). Did not perform a low velocity centrifugation to remove cells and cell particles.
3). The ratio is off. For example: 0.125 mL A + 0.125 mL B + 0.5mL C to 2 mL medium. Overproportion of serum/medium amount to Reply mixture (A/B/C) will end in an indistinctive interphase.
4). The utmost starting amount is 4 ml medium, and it should be from half million cultured cells or a lot much less. If cell amount or medium amount exceed this amount, the sample should be splits into a lot of samples to proceed.