Effective isolation of exosomes from plasma
General description
Effective isolation of exosomes from plasma
“Therefore, we looked at the ExoQuick method for further studies, as these samples required much less sample input, an important advantage when working with clinical samples and mouse models.” By isolating exosomes from plasma, fibrinogen and fibrin can prevent effective recovery.
By pre-treating plasma with thrombin, fibrinogen can be converted to fibrin and easily pre-clarified by a single centrifugation step. The resulting serum-like solution can now be treated with ExoQuick, the original formulation, for quantitative isolation of exosomes compatible with high flow methods.
ExoQuick fast, non-ultracentrifugation method:
- Save time and effort
- It is easily scalable
- Save a valuable sample
- It provides high yields of high quality and functional exosomes.
- It can be used to isolate exosomes for a wide range of downstream applications, including
- Biomarker studies
- Exosomal miRNA profiles
- Exosomal proteomics
- Lipidomics / exosomal metabolomics
- Functional studies, such as cell-to-cell signaling.
- Basic biology, such as its role in tumorigenesis.
ExoQuick is a proprietary polymer that gently precipitates exosomes. For isolation of exosomes from plasma, you can first defibrinize your sample with thrombin and then proceed with the standard ExoQuick protocol for pre-purifying your sample of cells and cell debris, adding the appropriate amount of ExoQuick, cooling, and then centrifuging (see product manual for protocol information). Your exosomes will be in the pellet, ready for resuspension in a suitable solution.
In electron microscopy studies, exosomes isolated with ExoQuick look like exosomes isolated by ultracentrifugation 1-2, and these exosomes are also active in numerous functional assays1-3.
Exosomes isolated with ExoQuick can be used for all types of protein profiling and characterization studies, such as mass spectrometry, Western blotting, ELISA and more. Higher protein yield is obtained by ExoQuick purification than by chromatography, DynaBeads or ultracentrifugation.
Exosomes isolated with ExoQuick also provide excellent samples for the study of exosome-associated nucleic acids, such as microRNAs, siRNAs, and even mRNAs. Quantitative analysis techniques such as qPCR, microarray studies, and next-generation sequencing are compatible with nucleic acids isolated from exosomes purified with ExoQuick.
Supported by an increasing number of publications, ExoQuick is often the best choice for researchers working with low sample volumes, such as clinical research samples or small animal models.
) Exosome Isolation kit (forHuman Body Fluid) |
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| P121S | 101Bio | - | Ask for price |
) Exosome Isolation kit (for cell culture media) |
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| P100 | 101Bio | - | Ask for price |
Exosome Isolation kit (for cell culture media) |
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| P100L | 101Bio | - | Ask for price |
Exosome Isolation kit (for cell culture media) |
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| P100S | 101Bio | - | Ask for price |
 Easy-Exo Saliva Exosome Isolation Kit, 95% pure |
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| P530 | 101Bio | - | Ask for price |
Easy-Exo Saliva Exosome Isolation Kit, 95% pure |
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| P530S | 101Bio | - | Ask for price |
) Exosome Isolation kit (for stem cell culture media) |
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| P107S | 101Bio | - | Ask for price |
Exosome Isolation kit (for stem cell culture media) |
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| P107 | 101Bio | - | Ask for price |
) VEX Exosome Isolation Reagent (from serum) |
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| R602 | Vazyme | 10 ml | EUR 769.2 |
) VEX Exosome Isolation Reagent (from plasma) |
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| R603 | Vazyme | 10 ml | EUR 769.2 |
) ExoPure? Reagent (Overall Exosome Isolation, urine) |
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| M1003-25 | Biovision | each | EUR 992.4 |
ExoPure? Reagent (Overall Exosome Isolation, urine) |
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| M1003-50 | Biovision | each | EUR 1083.6 |
) ExoPure? Reagent (Overall Exosome Isolation, cell media) |
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| M1002-25 | Biovision | each | EUR 992.4 |
ExoPure? Reagent (Overall Exosome Isolation, cell media) |
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| M1002-50 | Biovision | each | EUR 1083.6 |
) Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) |
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| P101EZ | 101Bio | - | Ask for price |
) VEX Exosome Isolation Reagent (from cell culture media) |
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| R601 | Vazyme | 50 ml | EUR 706.8 |
) ExoPure? Reagent (Overall Exosome Isolation, biological fluids) |
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| M1001-10 | Biovision | each | EUR 796.8 |
ExoPure? Reagent (Overall Exosome Isolation, biological fluids) |
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| M1001-20 | Biovision | each | EUR 1260 |
ExoPure? Reagent (Overall Exosome Isolation, biological fluids) |
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| M1001-5 | Biovision | each | EUR 633.6 |
) Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) |
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| P100EZ | 101Bio | - | Ask for price |
 ExoQuick-LP for lipoprotein pre-clear & exosome isolation |
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| EXOLP5A-1 | SBI | 5 reactions | EUR 473 |
 sample) Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample |
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| P101EZ--sample | 101Bio | - | Ask for price |
) ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml) |
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| EXOCG50A-1 | SBI | 50 ml | EUR 1106 |
) Reagent for Total Exosome Isolation (Culture Media Supplement) |
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| EIR-02 | Creative Biolabs | 50 ml | EUR 2485.2 |
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Description: This product is used for isolating exosomes from culture media supplement based on chemical precipitation isolation method. |
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) XCF COMPLETE Exosome and cfDNA Isolation Kit (for Serum and Plasma) |
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| XCF100A-1 | SBI | 20 rxn | EUR 583 |
 sample) Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample |
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| P100EZ-sample | 101Bio | - | Ask for price |
) TumorExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay) |
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| M1016-100-T | Biovision | each | EUR 874.8 |
) TumorExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay) |
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| M1017-100-W | Biovision | each | EUR 888 |
) TumorExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay) |
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| M1018-100-B | Biovision | each | EUR 888 |
) GlialExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay) |
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| M1022-100-T | Biovision | each | EUR 888 |
) GlialExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay) |
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| M1023-100-W | Biovision | each | EUR 888 |
) GlialExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay) |
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| M1024-100-B | Biovision | each | EUR 888 |
) ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma) |
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| EQ806A-1 | SBI | 20 preps | EUR 493 |
) NeuralExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay) |
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| M1019-100-T | Biovision | each | EUR 907.2 |
) NeuralExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay) |
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| M1020-100-W | Biovision | each | EUR 888 |
) NeuralExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay) |
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| M1021-100-B | Biovision | each | EUR 888 |
) ExoPure? Immunoplates (Overall Exosome Isolation, serum, colorimetric assay) |
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| M1007-100-T | Biovision | each | EUR 888 |
) ExoPure? Immunoplates (Overall Exosome Isolation, serum, luminometric assay) |
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| M1008-100-W | Biovision | each | EUR 888 |
) ExoPure? Immunoplates (Overall Exosome Isolation, serum, fluorimetric assay) |
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| M1009-100-B | Biovision | each | EUR 888 |
) ExoPure? Immunoplates (Overall Exosome Isolation, saliva, colorimetric assay) |
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| M1010-100-T | Biovision | each | EUR 888 |
) ExoPure? Immunoplates (Overall Exosome Isolation, saliva, luminometric assay) |
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| M1011-100-W | Biovision | each | EUR 888 |
) ExoPure? Immunoplates (Overall Exosome Isolation, saliva, fluorimetric assay) |
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| M1012-100-B | Biovision | each | EUR 888 |
ExoPure? Immunoplates (Overall Exosome Isolation, saliva, colorimetric assay) |
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| M1013-100-T | Biovision | each | EUR 888 |
ExoPure? Immunoplates (Overall Exosome Isolation, saliva, luminometric assay) |
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| M1014-100-W | Biovision | each | EUR 888 |
ExoPure? Immunoplates (Overall Exosome Isolation, saliva, fluorimetric assay) |
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| M1015-100-B | Biovision | each | EUR 888 |
) ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media) |
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| EQ806TC-1 | SBI | 10 preps | EUR 493 |
) ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum) |
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| M1038-10 | Biovision | each | EUR 1005.6 |
ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum) |
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| M1038-20 | Biovision | each | EUR 1546.8 |
ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum) |
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| M1038-3 | Biovision | each | EUR 523.2 |