Effective isolation of exosomes from plasma

exosome isolation kit

General description

Effective isolation of exosomes from plasma

“Therefore, we looked at the ExoQuick method for further studies, as these samples required much less sample input, an important advantage when working with clinical samples and mouse models.” By isolating exosomes from plasma, fibrinogen and fibrin can prevent effective recovery.

By pre-treating plasma with thrombin, fibrinogen can be converted to fibrin and easily pre-clarified by a single centrifugation step. The resulting serum-like solution can now be treated with ExoQuick, the original formulation, for quantitative isolation of exosomes compatible with high flow methods.

 

ExoQuick fast, non-ultracentrifugation method:

  • Save time and effort
  • It is easily scalable
  • Save a valuable sample
  • It provides high yields of high quality and functional exosomes.
  • It can be used to isolate exosomes for a wide range of downstream applications, including
  • Biomarker studies
  • Exosomal miRNA profiles
  • Exosomal proteomics
  • Lipidomics / exosomal metabolomics
  • Functional studies, such as cell-to-cell signaling.
  • Basic biology, such as its role in tumorigenesis.

ExoQuick is a proprietary polymer that gently precipitates exosomes. For isolation of exosomes from plasma, you can first defibrinize your sample with thrombin and then proceed with the standard ExoQuick protocol for pre-purifying your sample of cells and cell debris, adding the appropriate amount of ExoQuick, cooling, and then centrifuging (see product manual for protocol information). Your exosomes will be in the pellet, ready for resuspension in a suitable solution.

exosome isolation kit
exosome isolation kit

In electron microscopy studies, exosomes isolated with ExoQuick look like exosomes isolated by ultracentrifugation 1-2, and these exosomes are also active in numerous functional assays1-3.

Exosomes isolated with ExoQuick can be used for all types of protein profiling and characterization studies, such as mass spectrometry, Western blotting, ELISA and more. Higher protein yield is obtained by ExoQuick purification than by chromatography, DynaBeads or ultracentrifugation.

Exosomes isolated with ExoQuick also provide excellent samples for the study of exosome-associated nucleic acids, such as microRNAs, siRNAs, and even mRNAs. Quantitative analysis techniques such as qPCR, microarray studies, and next-generation sequencing are compatible with nucleic acids isolated from exosomes purified with ExoQuick.

Supported by an increasing number of publications, ExoQuick is often the best choice for researchers working with low sample volumes, such as clinical research samples or small animal models.

 

Exosome Isolation kit (forHuman Body Fluid)

P121S NULL
EUR 0

Exosome Isolation kit (for serum & plasma)

P101 NULL
EUR 0

Exosome Isolation kit (for serum & plasma)

P101L NULL
EUR 0

Exosome Isolation kit (for serum & plasma)

P101S NULL
EUR 0

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS NULL
EUR 0

Exosome Isolation kit (for cell culture media)

P100 NULL
EUR 0

Exosome Isolation kit (for cell culture media)

P100L NULL
EUR 0

Exosome Isolation kit (for cell culture media)

P100S NULL
EUR 0

ExoPure? Reagent (Overall Exosome Isolation, urine)

M1003-25
EUR 827

ExoPure? Reagent (Overall Exosome Isolation, urine)

M1003-50
EUR 903

VEX Exosome Isolation Reagent (from serum)

R602 10 ml
EUR 641

VEX Exosome Isolation Reagent (from plasma)

R603 10 ml
EUR 641

Easy-Exo Saliva Exosome Isolation Kit, 95% pure

P530 NULL
EUR 0

Easy-Exo Saliva Exosome Isolation Kit, 95% pure

P530S NULL
EUR 0

Exosome Isolation kit (for stem cell culture media)

P107 NULL
EUR 0

Exosome Isolation kit (for stem cell culture media)

P107S NULL
EUR 0

ExoPure? Reagent (Overall Exosome Isolation, biological fluids)

M1001-10
EUR 664

ExoPure? Reagent (Overall Exosome Isolation, biological fluids)

M1001-20
EUR 1050

ExoPure? Reagent (Overall Exosome Isolation, biological fluids)

M1001-5
EUR 528

ExoPure? Reagent (Overall Exosome Isolation, cell media)

M1002-25
EUR 827

ExoPure? Reagent (Overall Exosome Isolation, cell media)

M1002-50
EUR 903

TumorExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1016-100-T
EUR 729

TumorExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1017-100-W
EUR 740

TumorExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1018-100-B
EUR 740

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1019-100-T
EUR 756

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1020-100-W
EUR 740

NeuralExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1021-100-B
EUR 740

GlialExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1022-100-T
EUR 740

GlialExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1023-100-W
EUR 740

GlialExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1024-100-B
EUR 740

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome)

P101EZ NULL
EUR 0

Reagent for Total Exosome Isolation (Culture Media Supplement)

EIR-02 50 ml
EUR 2071
Description: This product is used for isolating exosomes from culture media supplement based on chemical precipitation isolation method.

ExoQuick-LP for lipoprotein pre-clear & exosome isolation

EXOLP5A-1 5 reactions
EUR 533
  • Category: Exosomes

ExoPure? Immunoplates (Overall Exosome Isolation, serum, colorimetric assay)

M1007-100-T
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, serum, luminometric assay)

M1008-100-W
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, serum, fluorimetric assay)

M1009-100-B
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, colorimetric assay)

M1010-100-T
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, luminometric assay)

M1011-100-W
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, fluorimetric assay)

M1012-100-B
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, colorimetric assay)

M1013-100-T
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, luminometric assay)

M1014-100-W
EUR 740

ExoPure? Immunoplates (Overall Exosome Isolation, saliva, fluorimetric assay)

M1015-100-B
EUR 740

Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, colorimetric assay)

M1025-100-T
EUR 740

Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, luminometric assay)

M1026-100-W
EUR 740

Monocytes/PlateletExoPure? Immunoplates (Exosome Isolation, plasma, fluorimetric assay)

M1027-100-B
EUR 740

VEX Exosome Isolation Reagent (from cell culture media)

R601 50 ml
EUR 589

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome)

P100EZ NULL
EUR 0

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample

P101EZ--sample NULL
EUR 0

ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)

EXOCG50A-1 50 ml
EUR 1181
  • Category: Exosomes