Cultured Cells Epigentek Chromoplasts Chromatin Extraction Kit
Background Information
Chromatin immunoprecipitaton (ChIP) offers an advantageous gadget for locating out protein-DNA interaction. With ChIP, the experimenter can resolve if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies.
For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, the ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly demanded.
Product Overview
The ChromaFlash Chromatin Extraction Package deal is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle could be utilized in various chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Package deal. The isolated chromatin may also be utilized in several chromatin-related features equal to in vitro protein-DNA binding assays and nuclear enzyme assays.
- Terribly fast course of: your complete course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
- Helpful and versatile: the bundle is acceptable for preparing every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
- Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.
Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, up to date and frozen tissues, and so forth. The amount of cells and tissues for each preparation might be 1 x 105 to 5 x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues. Yield of chromatin is roughly 4 ug per 106 cells or per 50 mg tissues.
Background Information
Chromatin immunoprecipitation (ChIP) offers an advantageous gadget for locating out protein-DNA interaction. With ChIP, the experimenter can resolve if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, a ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly in demand.
 miRNA Extraction Kit |
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| K1456-50 | Biovision | 50 Preps | EUR 551 |
 Membrane Protein Extraction Kit |
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| 55R-1357 | Fitzgerald | 50 assays | EUR 740 |
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Description: Membrane Protein Extraction Kit for use in the research laboratory |
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 Mammalian Cell Extraction Kit |
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| 55R-1358 | Fitzgerald | 500 assays | EUR 381 |
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Description: Mammalian Cell Extraction Kit for use in the research laboratory |
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 Quick Gel Extraction Kit |
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| 20-abx098085 | Abbexa |
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 Up RNA Extraction Kit |
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| 20-abx098087 | Abbexa |
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 Plant RNA extraction Kit |
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| 20-abx098088 | Abbexa |
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 Genomic DNA Extraction Kit |
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| K5016005 | Biochain | 1 kit | EUR 329 |
Membrane Protein Extraction Kit |
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| K268-50 | Biovision | EUR 539 | |
Mammalian Cell Extraction Kit |
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| K269-500 | Biovision | EUR 294 | |
 Total RNA Extraction Kit |
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| K2014005 | Biochain | 1 kit | EUR 344 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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 Plant Tissue Extraction Kit |
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| K296-50 | Biovision | EUR 457 | |
 Total Protein Extraction Kit |
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| K3011010 | Biochain | 1 kit | EUR 370 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Membrane Protein Extraction Kit |
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| K3014005 | Biochain | 1 kit | EUR 382 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Membrane Protein Extraction Kit |
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| K2113-50 | ApexBio | 50 assays | EUR 544 |
Mammalian Cell Extraction Kit |
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| K2114-500 | ApexBio | 500 assays | EUR 321 |
 Exosomal RNA Extraction Kit |
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| P200R,-part-of-P200 | 101Bio | NULL | EUR 0 |
 Exosomal DNA Extraction Kit |
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| P230-25 | 101Bio | NULL | EUR 0 |
Exosomal DNA Extraction Kit |
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| P230-50 | 101Bio | NULL | EUR 0 |
 EpiQuik Nuclear Extraction Kit |
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| OP-0002 | EpiGentek | 100 Assays | EUR 292.15 |
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Description: kits suitable for this type of research |
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 Bacterial RNA Extraction Kit |
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| R403-01 | Vazyme | 100 rxn | EUR 155 |
Total Protein Extraction Kit |
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| BSP003 | Bio Basic | 50Preps | EUR 109.16 |
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 Plant Protein Extraction Kit |
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| BSP004 | Bio Basic | 20Preps | EUR 110.9 |
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 Nuclear Protein Extraction Kit |
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| BSP009 | Bio Basic | 50Preps | EUR 89.15 |
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 FFPE Protein Extraction Kit |
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| BSP058 | Bio Basic | 50Preps | EUR 102.2 |
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 Frit Kit |
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| FRIT-KIT | Next Advance | 1each | EUR 124 |
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Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
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 Column Packing Kit |
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| PACK-KIT | Next Advance | 1pack | EUR 1035 |
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Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
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, for Histon Extraction) Triton Extraction Buffer(TEB), for Histon Extraction |
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| T8300-100 | GenDepot | 100ml | EUR 126 |
Triton Extraction Buffer(TEB), for Histon Extraction |
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| T8300-500 | GenDepot | 500ml | EUR 142 |
 PCR Mycoplasma Detection Kit |
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| M034-Kit | TOKU-E | Kit | EUR 266 |
 EpiQuik Chromatin Accessibility Assay Kit |
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| P-1047 | EpiGentek | 48 Assays | EUR 759.05 |
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Description: Ask the seller for details |
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 Kit) EpiQuik Chromatin Immunoprecipitation (ChIP) Kit |
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| P-2002 | EpiGentek | 96 Reactions | EUR 904.05 |
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Description: Ask the seller for details |
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 ChromaFlash Chromatin Isolation & Shearing Kit |
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| P-2023 | EpiGentek | 100 Preperations | EUR 270.4 |
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Description: kits suitable for this type of research |
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 Mammalian Membrane Protein Extraction Kit |
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| AR0155 | BosterBio | 1 kit | EUR 152 |
 DNA Extraction Kit in Tubes |
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| D100T | Cygnus Technologies | 1 kit (50 tubes) | EUR 469 |
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Description: DNA Extraction Kit in Tubes by Cygnus Technologies is available in Europe via Gentaur. |
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 DNA Extraction Kit in Wells |
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| D100W | Cygnus Technologies | 1 kit (96 wells plate) | EUR 469 |
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Description: DNA Extraction Kit in Wells by Cygnus Technologies is available in Europe via Gentaur. |
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 Up Plus RNA Extraction Kit |
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| 20-abx098093 | Abbexa |
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 Mammalian Total Protein Extraction Kit |
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| 20-abx098853 | Abbexa |
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Mammalian Membrane Protein Extraction Kit |
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| 20-abx098855 | Abbexa |
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 Xpert Total Histone Extraction Kit |
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| H7200-050 | GenDepot | 50 ext | EUR 361 |
Xpert Total Histone Extraction Kit |
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| H7200-100 | GenDepot | 100 ext | EUR 509 |
 Small DNA Fragment Extraction Kit |
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| FYG203-100P | Yeastern Biotech | 100 Preps | Ask for price |
Small DNA Fragment Extraction Kit |
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| FYG203-300P | Yeastern Biotech | 300 Preps | Ask for price |