bt3technologies

Convenient Agarose Preparation with Hydrogen Peroxide and Desulfation Process Analysis

Agarose is a pure seaweed polysaccharide and broadly used within the medication, meals, and organic fields due to its excessive gel power, non-toxicity, and electrical neutrality. The sulfate group is without doubt one of the essential charged teams that have an effect on the efficiency of agarose. Within the current examine, a easy, eco-friendly, and environment friendly methodology was explored for agarose preparation. After desulfation with hydrogen peroxide (H2O2), the sulfate content material of agar reached 0.21%. Along with gel power, electroendosmosis, gelling and melting temperature, the indications of desulfated agar met the requirements of commercially obtainable agarose. Notably, the desulfated agar can be utilized as an agarose gel electrophoresis medium to separate DNA molecules, and the separation impact is pretty much as good as that of commercially obtainable agarose.
Additional, the H2O2 desulfation course of was analyzed. The addition of a hydroxyl radical (HO•) scavenger remarkably decreased the H2O2 desulfation price, indicating that HO• has a sure function in agar desulfation. Sulfate content material detection indicated that sulfur was faraway from agar molecules within the type of sulfate ions (SO42-) and steel sulfate. The band absence at 850 cm-1 indicated that the sulfate teams at C-Four of D-galactose in sulfated galactan had been eradicated.

Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Tradition

Three-dimensional (3D) bioprinting makes use of hydrogel-based composites (or biomaterial inks) which are deposited in a sample, forming a substrate onto which cells are deposited. As a result of many biomaterial inks might be probably cytotoxic to major cells, it’s needed to find out the biocompatibility of those hydrogel composites previous to their utilization in expensive 3D tissue engineering processes. Some 3D tradition strategies, together with bioprinting, require that cells be embedded right into a 3D matrix, making it tough to extract and analyze the cells for adjustments in viability and biomarker expression with out eliciting mechanical harm.
This protocol describes as proof of idea, a way to evaluate the biocompatibility of a crystalline nanocellulose (CNC) embedded agarose composite, fabricated right into a 24-well tradition system, with mouse bone marrow-derived mast cells (BMMCs) utilizing movement cytometric assays for cell viability and biomarker expression. After 18 h of publicity to the CNC/agarose/D-mannitol matrix, BMMC viability was unaltered as measured by propidium iodide (PI) permeability.
Nonetheless, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to barely improve their expression of the high-affinity IgE receptor (FcεRI) and the stem cell issue receptor (Package; CD117), though this doesn’t seem like depending on the quantity of CNC within the bioink composite. The viability of BMMCs was additionally assessed following a time course publicity to hydrogel scaffolds that had been fabricated from a industrial biomaterial ink composed of fibrillar nanocellulose (FNC) and sodium alginate utilizing a 3D extrusion bioprinter.
Over a interval of 6-48 h, the FNC/alginate substrates didn’t adversely have an effect on the viability of the BMMCs as decided by movement cytometry and microtiter assays (XTT and lactate dehydrogenase). This protocol describes an environment friendly methodology to quickly display screen the biochemical compatibility of candidate biomaterial inks for his or her utility as 3D scaffolds for post-print seeding with mast cells.

Controlling Floor-Induced Platelet Activation by Agarose and Gelatin-Primarily based Hydrogel Movies

Platelet-surface interplay is of paramount significance in biomedical purposes in addition to in vitro research. Nonetheless, controlling platelet-surface activation is difficult and nonetheless requires extra effort as they activate instantly when contacting with any nonphysiological floor. As hydrogels are extremely biocompatible, on this examine, we developed agarose and gelatin-based hydrogel movies to inhibit platelet-surface adhesion.
We discovered promising agarose movies that exhibit greater floor wettability, higher controlled-swelling properties, and better stiffness in comparison with gelatin, leading to a robust discount of platelet adhesion. Mechanical properties and floor wettability of the hydrogel movies had been diverse by including magnetite (Fe3O4) nanoparticles.
Whereas the entire movies prevented platelet spreading, movies shaped by agarose and its nanocomposite repelled platelets and inhibited platelet adhesion and activation stronger than these of gelatin. Our outcomes confirmed that platelet-surface activation is modulated by controlling the properties of the movies beneath platelets and that the bioinert agarose might be probably translated to the event of platelet storage and different medical purposes.

Agarose, Alginate and Chitosan Nanostructured Aerogels for Pharmaceutical Functions: A Brief Overview

 

On this brief evaluate, drug supply methods, shaped by polysaccharide-based (i.e., agarose, alginate, and chitosan) aerogels, are analyzed. Specifically, the principle papers, printed within the interval 2011-2020 on this analysis subject, have been investigated and critically mentioned, in an effort to spotlight strengths and weaknesses of the normal manufacturing methods (e.g., freeze-drying and air evaporation) of bio-aerogels with respect to supercritical CO2 assisted drying.
Supercritical CO2 assisted drying demonstrated to be a promising method to supply nanostructured bio-aerogels that preserve the beginning gel quantity and form, when the solvent elimination happens at negligible floor stress. This attribute, coupled with the potential of eradicating additionally cross-linking agent residues from the aerogels, makes these superior units secure and appropriate as carriers for managed drug supply purposes.

Low bias a number of displacement amplification with confinement impact primarily based on agarose gel

A number of displacement amplification (MDA) is a well-liked single-cell whole-genome amplification (WGA) method that may vastly enhance the amplification effectivity of single-cell genomes. Nonetheless, there may be an inherent drawback that can’t be fully solved, that’s, the amplification bias. We right here suggest an improved MDA methodology primarily based on low melting agarose gel, named gelMDA. Firstly, the agarose gel and resolution had been characterised with SEM and fluorescent reagent.
Then, we used gelMDA for cDNA amplification in library preparation of RNA-seq, and traditional MDA was used as a comparability. The sensitivity, effectivity of gelMDA, and amplification bias had been evaluated with fluorescence curve, product yield, and the sequencing outcomes. Lastly, gelMDA was used for single-cell transcriptome sequencing. The outcomes confirmed that the sensitivity and product yield of gelMDA had been considerably greater than these of typical MDA.
A decrease coefficient of variation (CV) and a better reproducibility had been obtained from gelMDA sequencing outcomes. A area of 30 μm in diameter was amplified from the tissue sections and efficiently sequenced. In conclusion, gelMDA obtained greater amplification effectivity and decrease amplification bias within the current examine. It advised the good potential in single-cell RNA amplification and sequencing.

General Purpose Sample Diluent-SD1

KF17352 500 ml
EUR 291

Sample Diluent

I094 1000 ml
EUR 519
Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Sample Diluent

I094-100 100 ml
EUR 211
Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Sample Diluent

I094-500 500 ml
EUR 373
Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Conjugate Diluent

I095 1 L
EUR 565
Description: Conjugate Diluent by Cygnus Technologies is available in Europe via Gentaur.

Antibody Diluent

AR1016 100mL (for 10000-15000 assays)
EUR 131

Diluent A

abx098961-6ml 6 ml
EUR 91

Diluent B

abx098962-12ml 12 ml
EUR 91

EIA Diluent

2001-100ml 100 ml
EUR 282

EIA Diluent

2001-1L 1 Liter
EUR 1181

HRP Diluent

85-H10S 250 ml
EUR 300
Description: Dilution and stabilization buffer for monoclonal or polyclonal antibody HRP conjugates

Sample Diluent, 60ML

X028-60ML 60ML
EUR 143

NADPH Diluent, 5ML

X034-5ML 5ML
EUR 87

Conjugate Diluent, 13ML

X076-13ML 13ML
EUR 122

Conjugate Diluent, 3ML

X076-3ML 3ML
EUR 80

Substrate Diluent , 12ML

X101-12ML 12ML
EUR 128

BSA Sample Diluent

F031A 1000 ml
EUR 488
Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

BSA Sample Diluent

F031A-100 100 ml
EUR 195
Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

BSA Sample Diluent

F031A-500 500 ml
EUR 411
Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Sample Diluent Buffer

I028-100 100 ml
EUR 211
Description: Sample Diluent Buffer by Cygnus Technologies is available in Europe via Gentaur.

Sample Diluent Buffer

I028-1000 1000 ml
EUR 519
Description: Sample Diluent Buffer by Cygnus Technologies is available in Europe via Gentaur.

Sample Diluent Buffer

I028-500 500 ml
EUR 373
Description: Sample Diluent Buffer by Cygnus Technologies is available in Europe via Gentaur.

HEPES Sample Diluent

I050 1000 ml
EUR 527
Description: HEPES Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

HEPES Sample Diluent

I050-100 100 ml
EUR 219
Description: HEPES Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

HEPES Sample Diluent

I050-500 500 ml
EUR 334
Description: HEPES Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Conjugate Diluent/HRP

I056 1000 ml
EUR 565
Description: Conjugate Diluent/HRP by Cygnus Technologies is available in Europe via Gentaur.

DNA Sample Diluent

D006-100 100 ml
EUR 373
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Sample Diluent

D006-1000 1000 ml
EUR 1167
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Sample Diluent

D006-500 500 ml
EUR 726
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

TissueDigest Diluent, 10X

T102 10ml
EUR 94

Sample diluent buffer

AR1106-1 30ml
EUR 91

Antibody diluent buffer

AR1106-2 12ml
EUR 91

ABC diluent buffer

AR1106-3 12ml
EUR 91

Standard Diluent Buffer

abx098951-20ml 20 ml
EUR 91

Sample Diluent Buffer

abx296001-20ml 20 ml
EUR 91

Diluent Buffer A

abx296005-10ml 10 ml
EUR 91

ELISA Sample Diluent

ESD125 125 ml
EUR 80

ELISA Sample Diluent

ESD500 500 ml
EUR 109

ELISA Sample Diluent

ESD999 1000 ml
EUR 157

Enzyme Label Diluent

ABI500 500 ml
EUR 101

Enzyme Label Diluent

ABI999 1000 ml
EUR 124

NHE-BSA Diluent

1Z0216-100ml 100 ml
EUR 231

NHE-BSA Diluent

1Z0216-1L 1 Liter
EUR 956

TMB Substrate Diluent

42R-TB105 1 liter
EUR 300
Description: Dilution buffer for TMB Substrate in immunoassays

Antibody Diluent Kit

GR103015 100 mL
EUR 68

EpiQuik General Protein-DNA Binding Assay Kit (Colorimetric)

P-2004 96 Assays
EUR 614.05
Description: kits suitable for this type of research

EpiQuik General Protein-DNA Binding Assay Kit (Fluorometric)

P-2005 96 Assays
EUR 628.55
Description: Ask the seller for details

Hemoglobin Sample Diluent, 50ML

X052-50ML 50ML
EUR 153

Sample Diluent (Conc.), 12ML

X074-12ML 12ML
EUR 123

Sample Diluent (Conc.), 60ML

X074-60ML 60ML
EUR 143

SP2/0 Sample Diluent

F183A 1000 ml
EUR 835
Description: SP2/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

SP2/0 Sample Diluent

F183A-100 100 ml
EUR 257
Description: SP2/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

SP2/0 Sample Diluent

F183A-500 500 ml
EUR 488
Description: SP2/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Goat IgG Sample Diluent

F213A 1000 ml
EUR 565
Description: Goat IgG Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Goat IgG Sample Diluent

F213A-100 100 ml
EUR 257
Description: Goat IgG Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Goat IgG Sample Diluent

F213A-500 500 ml
EUR 411
Description: Goat IgG Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

NS/0 Sample Diluent

F223A 1000 ml
EUR 835
Description: NS/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

NS/0 Sample Diluent

F223A-100 100 ml
EUR 257
Description: NS/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

NS/0 Sample Diluent

F223A-500 500 ml
EUR 488
Description: NS/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

A549 Sample Diluent, 100mL

F233A-100 100 ml
EUR 257
Description: A549 Sample Diluent, 100mL by Cygnus Technologies is available in Europe via Gentaur.

A549 Sample Diluent, 500mL

F233A-500 500 ml
EUR 411
Description: A549 Sample Diluent, 500mL by Cygnus Technologies is available in Europe via Gentaur.

450ML SequaGel UreaGel Diluent

NAT1152 450ML
EUR 75

1L SequaGel UreaGel Diluent

NAT1154 1L
EUR 86

Neptune Sample Diluent-SD3

KF17354 500 ml
EUR 244

cDNA Probe Diluent Solution

AR0063 5mL
EUR 106

HRP Conjugate Stabilizer Diluent

abx090812-1000ml 1000 ml
EUR 662

Alk-Phos Stabilizing Diluent

APD500 500 ml
EUR 119

Alk-Phos Stabilizing Diluent

APD999 1000 ml
EUR 165

DB Primary Antibody diluent

DBD-125 125 ml
EUR 150

DB Primary Antibody diluent

DBD-250 250 ml
EUR 245

DB Primary Antibody diluent

DB-D-125 125 ml
EUR 150

DB Primary Antibody diluent

DB-D-250 250 ml
EUR 245

Rheumatoid Factor Control Diluent

35-RC15 100 ml
EUR 627
Description: Rheumatoid Factor Control Diluent

Universal Casein Diluent/Blocker

85R-108 250 ml
EUR 230
Description: Ready-to-use biotin free reagent applicable for blocking ELISA plates

Whole egg yolk (IgY) sample/Conjugate Diluent (Green) for ELISA (antibody assay) concentrate (10X)

80071 50 ml
EUR 202

Goat Milk Proteins Sample Diluent

F243A 1000 ml
EUR 565
Description: Goat Milk Proteins Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Goat Milk Proteins Sample Diluent

F243A-100 100 ml
EUR 257
Description: Goat Milk Proteins Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Goat Milk Proteins Sample Diluent

F243A-500 500 ml
EUR 411
Description: Goat Milk Proteins Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Mix-N-Go Sample Diluent

I600 25 ml
EUR 180
Description: Mix-N-Go Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Sample Diluent Buffer, 25 mL

SDB-025 25 mL
EUR 121.34
Description: A buffer for dilution of samples in a lateral flow assay. It is for 250 samples.

HRP Stabilizing Diluent, Phosphate Buffered

H3210-010 100ml
EUR 131

HRP Stabilizing Diluent, Phosphate Buffered

H3210-050 500ml
EUR 232

Standard and Sample Diluent Buffer

abx092103-20ml 20 ml
EUR 91

HRP Stabilizing Diluent (Phosphate Buffered)

HSB500 500 ml
EUR 109

HRP Stabilizing Diluent (Phosphate Buffered)

HSB999 1000 ml
EUR 157

HRP Stabilizing Diluent (MOPS Buffered)

HSM125 125 ml
EUR 84

HRP Stabilizing Diluent (MOPS Buffered)

HSM500 500 ml
EUR 109

HRP Stabilizing Diluent (MOPS Buffered)

HSM999 1000 ml
EUR 157