2 Apb 4-Hne Antibody Arf1 Antibody azide free Cdc20 Antibody Elisa Array Helicobacter histo Human Influenza Kd Antibody Kdel Antibody Mink Mouse Opossum Orangutan Rhcg Antibody What Are Feeder Cells
Activated-carbon-filled agarose hydrogel as a natural medium for seed germination and seedling growth
An activated-carbon-filled agarose (Agar-AC) hydrogel containing numerous concentrations of activated carbon (AC) was efficiently fabricated by a easy solvent solid approach. In comparison with pure agarose hydrogels, Agar-AC hydrogels exhibited glorious mechanical properties, good thermal stability and a extremely developed pore construction. The Agar-AC hydrogels additionally confirmed a sure diploma of enchancment in water retention efficiency, whereas their swelling ratio decreased with the addition of AC.
The incorporation of AC didn’t affect the crystallinity of the agarose hydrogel, and no chemical modification occurred in keeping with XRD and FTIR. In rapeseed seed germination experiments, the expansion indexes of rapeseed, together with the germination proportion, root size, stem size, recent weight and dry weight, had been enhanced by the incorporation of an appropriate quantity of AC. These outcomes indicated that AC has nice potential to boost the properties of agarose hydrogels and enhance seed germination and plant development, which suggests that Agar-AC hydrogels can be utilized as pure supplies for agricultural functions.
Impact of amine size within the interference of the multipoint covalent immobilization of enzymes on glyoxyl agarose beads
Trypsin, chymotrypsin, penicillin G acylase and ficin extract have been stabilized by immobilization on glyoxyl agarose, including completely different aliphatic compounds bearing a main amine group throughout the immobilization: ethyl amine, butyl amine, hexyl amine (at concentrations starting from Zero to 20 mM) and octyl amine (from Zero to 10 mM) to research their results on the immobilized enzyme stability. As anticipated, the presence of amines lowered the depth of the enzyme-support multipoint covalent attachment, and due to this fact the enzyme stability.
Nevertheless, it’s clear that this impact is larger utilizing octyl amine for all enzymes (in some circumstances the enzyme immobilized within the presence of 10 mM octyl amine was virtually inactivated whereas the reference stored over 50% of the preliminary exercise). This manner, plainly crucial impact of the presence of aminated compounds got here from the technology of steric hindrances to the enzyme/help multi-reaction promoted by the ammines which can be interacting with the aldehyde teams.
In some situations, simply 1 mM of aminated compounds is sufficient to tremendously lower enzyme stability. The outcomes recommended that, if the composition of the enzyme extract is unknown, to eradicate small aminated compounds could also be needed to maximise the enzyme-support response.
Hydrogen Bond Interpenetrated Agarose/PVA Community: A Extremely Ionic Conductive and Flame-Retardant Gel Polymer Electrolyte
The gel polymer electrolyte (GPE) is the important thing to assembling high-performance solid-state supercapacitors (SSCs). The industrial poly(vinyl alcohol) (PVA) GPE has developed a status because of low ionic conductivity endowed by its excessive crystallinity and poor water retention capability. On this work, density useful concept (DFT) calculations first revealed that the excessive crystallinity of PVA could be tremendously disrupted by forming hydrogen bonds with pure agarose macromolecules.
The hydrogen bond interpenetrated three-dimensional agarose/PVA community gives excessive water retention and huge quantities of channels for motion of Li+ on hydroxyl oxygen atoms. So, an optimized formation of the Li-O coordinate bond (gLi-O(r) = 8.78) and improved diffusion coefficient of Li+ (DLi+) (71 × 10-6 cm2 s-1) had been obtained within the agarose/PVA mannequin.
When assembled into SSCs, agarose/PVA-GPE with 2 M LiOAc (AP-GPE) reveals an excellent particular capacitance (697.22 mF cm-2 at 5 mA cm-2). The excessive water retention of agarose and huge quantities of -OH teams within the agarose macromolecular can generate H2O by dehydration response, lowering the flammability of PVA and tremendously enhancing the protection of SSCs.
Agarose-resolvable InDel markers primarily based on entire genome re-sequencing in cucumber
Insertion and Deletion (InDel) are widespread options in genomes and are related to genetic variation. The entire-genome re-sequencing knowledge from two dad and mom (X1 and X2) of the elite cucumber (Cucumis sativus) hybrid selection Lvmei No.1 was used for genome-wide InDel polymorphisms evaluation. Obtained sequence reads had been mapped to the genome reference sequence of Chinese language recent market sort inbred line ‘9930’ and gaps conforming to InDel had been pinpointed.
Additional, the extent of cross-parents polymorphism amongst 5 pairs of cucumber breeding dad and mom and their corresponding hybrid varieties had been used for evaluating hybrid seeds purity take a look at effectivity of InDel markers. A panel of 48 cucumber breeding traces was utilized for PCR amplification versatility and phylogenetic evaluation of those markers. In complete, 10,470 candidate InDel markers had been recognized for X1 and X2. Amongst these, 385 markers with greater than 30 nucleotide distinction had been arbitrary chosen. These markers had been chosen for experimental resolvability by electrophoresis on an Agarose gel.
2 hundred and eleven (211) accounting for 54.81% of markers may very well be validated as single and clear polymorphic sample whereas 174 (45.19%) confirmed unclear or monomorphic genetic bands between X1 and X2. Cross-parents polymorphism analysis recorded 68 (32.23%) of those markers, which had been designated as cross-parents transferable (CPT) InDel markers.
Apparently, the marker InDel114 offered experimental transferability between cucumber and melon. A panel of 48 cucumber breeding traces together with dad and mom of Lvmei No. 1 subjected to PCR amplification versatility utilizing CPT InDel markers efficiently clustered them into fruit and customary cucumber varieties primarily based on phylogenetic evaluation. It’s price noting that 16 of those markers had been predominately related to enzymatic actions in cucumber. These agarose-based InDel markers may represent a useful useful resource for hybrid seeds purity testing, germplasm classification and marker-assisted breeding in cucumber.
Single step immobilization of CMCase inside agarose gel matrix: Kinetics and thermodynamic research
Within the present research, CMCase from Bacillus licheniformis KIBGE-IB2 was immobilized throughout the matrix of agarose gel by entrapment approach. Most immobilization yield (%) of the enzyme was obtained when 2.0 % agarose was used. The activation vitality (Ea) of the enzyme elevated from 16.38 to 44.08 kJ mol-1 after immobilization.
Thermodynamic parameters reminiscent of activation vitality of deactivation (ΔGd), enthalpy (ΔHd) and entropy (ΔSd) of deactivation, deactivation fee fixed (Okd), half-life (thalf), D-value and z-value had been calculated for native/free and immobilized CMCase. The utmost response fee (Vmax) of the native enzyme was discovered to be 8319.47 U ml-1 min-1, which lowered to 7218.1 U ml-1 min-1after immobilization course of.
Nevertheless, the Michaelis-Menten fixed (Okm) worth of the enzyme elevated from 1.236 to 2.769 mg ml-1 min-1 after immobilization. Immobilized enzyme inside agarose gel matrix help could be reuse as much as eight response cycles. Broad stability profile and improved catalytic properties of the immobilized CMCase indicated that this enzyme is usually a believable candidate for use in numerous industrial processes.
 FAM-Spacer-Phe-CMK FLISP™ Assay Kit |
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| KF17323 | Neuromics | 100 Tests | EUR 834 |
 FAM-Leu-CMK FLISP™ Assay Kit |
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| KF17317 | Neuromics | 100 Tests | EUR 834 |
 FAM-Phe-CMK FLISP™ Assay Kit |
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| KF17314 | Neuromics | 25 Tests | EUR 349.2 |
FAM-Phe-CMK FLISP™ Assay Kit |
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| KF17315 | Neuromics | 100 Tests | EUR 834 |
 FAM-Lue-CMK FLISP™ Assay Kit |
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| KF17316 | Neuromics | 25 Tests | EUR 349.2 |
 SR-101-Leu-CMK FLISP™ Assay Kit |
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| KF17321 | Neuromics | 100 Tests | EUR 834 |
 FAM-Leu-CMK Serine Protease Assay Kit |
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| abx299011-25tests | Abbexa | 25 tests | EUR 679.2 |
 SR-101-Phe-CMK FLISP™ Assay Kit |
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| KF17318 | Neuromics | 25 Tests | EUR 349.2 |
SR-101-Phe-CMK FLISP™ Assay Kit |
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| KF17319 | Neuromics | 100 Tests | EUR 834 |
 FAM-Phe-CMK Serine Protease Assay Kit |
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| abx299010-25tests | Abbexa | 25 tests | EUR 679.2 |
 FAM-Leu-DAP Serine Protease Assay Kit |
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| abx299012-25tests | Abbexa | 25 tests | EUR 679.2 |
) OPTIONAL GREEN FILTER (572NM) |
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| GDBL-GREEN | CORNING | 1/pk | EUR 1262.4 |
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Description: Lab Equipment; Axygen Branded EQ |
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 Polycaspase Assay Kit, green |
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| KF17200 | Neuromics | 25 Tests | EUR 364.8 |
 FAM-Phe-DAP Serine Protease Assay Kit |
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| abx299013-25tests | Abbexa | 25 tests | EUR 698.4 |
 Malachite Green Phosphate Assay Kit |
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| abx298878-100Assays | Abbexa | 100 Assays | EUR 453.6 |
 Caspase 1 Assay Kit, green |
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| KF17201 | Neuromics | 25 Tests | EUR 390 |
 Caspase 2 Assay Kit, green |
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| KF17202 | Neuromics | 25 Tests | EUR 364.8 |
 Caspase 6 Assay Kit, green |
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| KF17204 | Neuromics | 25 Tests | EUR 364.8 |
 Caspase 8 Assay Kit, green |
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| KF17205 | Neuromics | 25 Tests | EUR 364.8 |
 Caspase 9 Assay Kit, green |
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| KF17206 | Neuromics | 25 Tests | EUR 364.8 |
 Caspase 10 Assay Kit, green |
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| KF17207 | Neuromics | 25 Tests | EUR 364.8 |
 Caspase 13 Assay Kit, green |
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| KF17208 | Neuromics | 25 Tests | EUR 364.8 |
Malachite Green Phosphate Assay Kit |
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| POMG-25H | BioAssay Systems | 2500 | EUR 330 |
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Description: High-throµghput phosphate assay using malachite green method at 620nm. Kit size: 2500 tests. Detection limit: 0.02 µM. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C. |
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Malachite Green Phosphate Assay Kit |
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| Z5030012 | Biochain | 2,500 assays | EUR 410.4 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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 Malachite Green Phosphate Assay kit |
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| 65-POMG-25H | Gentaur Genprice | 2500 tests | EUR 333 |
) HDAC Fluorogenic Assay Kit (Green) |
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| 50034 | BPS Bioscience | 96 rxns. | EUR 450 |
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Description: The Fluorogenic HDAC Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) class 1 activity for screening_x000D_and profiling applications. The kit comes in a convenient 96-well format, with all the_x000D_reagents necessary for 100 fluorescent HDAC activity measurements. In_x000D_addition, the kit includes purified HDAC2 enzyme and a potent HDAC inhibitor,_x000D_Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC_x000D_Assay Kit is based on a unique fluorogenic substrate and developer combination._x000D_This assay method eliminates dealing with the radioactivity, extraction, and_x000D_chromatography aspects of traditional assays. Using this kit, only two simple_x000D_steps on a microtiter plate are needed to analyze the HDAC activity level. First,_x000D_the HDAC fluorometric substrate, containing an acetylated lysine side chain, is_x000D_incubated with purified HDAC enzyme. The deacetylation sensitizes the_x000D_substrate so subsequent treatment with the Lysine Developer produces a_x000D_fluorophore that can then be measured using a fluorescence reader at 485 nm_x000D_(excitation)/528 nm (emission)._x000D__x000D_Our other HDAC kit (#50033) contains a substrate that is excited at wavelengths 350-380 nm and fluoresces at wavelengths 440-460 nm. However, the substrate in this kit fluoresces at longer (green) wavelengths: 485 nm(excitation)/528 nm (emission). It is most useful when the sample or inhibitor fluoresces at wavelengths that overlap those of our other HDAC substrate |
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 FAM-cAMP PDE IV substrate *Green Fluorescence* |
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| 13602 | AAT Bioquest | 0.5 umol | EUR 367.2 |
 FAM-cGMP PDE V substrate *Green Fluorescence* |
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| 13604 | AAT Bioquest | 0.5 umol | EUR 367.2 |
 Morpheus HT-96 Green Screen |
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| M-MD1-47-GREEN | MiTeGen | 96 x 1 ml ml | EUR 280 |
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Description: Morpheus HT-96 Green Screen |
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 Caspase 3 & 7 Assay Kit, green |
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| KF17203 | Neuromics | 25 Tests | EUR 364.8 |
) EZCell? Phagocytosis Assay Kit (Green Zymosan) |
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| K397-100 | Biovision | each | EUR 516 |
 Pesticide Kit Containing All 10 Multi-Compound Mixes |
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| SPXPR-KIT | Scientific Laboratory Supplies | 1ML | EUR 1923.18 |
 Puck Spacer |
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| M-CBP-PSP1 | MiTeGen | 1 UNIT | EUR 133 |
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Description: Puck Spacer |
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 Styrofoam Spacer |
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| M-CP-111-009S | MiTeGen | 1 UNIT | EUR 6 |
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Description: Styrofoam Spacer |
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) OxiSelect Intracellular ROS Assay Kit (Green Fluorescence) |
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| STA-342 | Cell Biolabs | 96 assays | EUR 762 |
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Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader. |
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OxiSelect Intracellular ROS Assay Kit (Green Fluorescence) |
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| STA-342-5 | Cell Biolabs | 5 x 96 assays | EUR 3058.8 |
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Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader. |
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) OxiSelect Cellular Antioxidant Assay Kit (Green Fluorescence) |
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| STA-349 | Cell Biolabs | 192 assays | EUR 644.4 |
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Description: The OxiSelect Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells. Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested. After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator. The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF. The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner. |
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) EZCell? Phagocytosis Assay Kit (Green E. coli) |
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| K963-100 | Biovision | each | EUR 516 |
 Cell Meter™ Autophagy Assay Kit *Green Fluorescence* |
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| 23002 | AAT Bioquest | 200 Tests | EUR 367.2 |
 Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence* |
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| 11401 | AAT Bioquest | 200 Tests | EUR 262.8 |
 Amplite™ Fluorimetric Glutathione Assay Kit *Green Fluorescence* |
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| 10055 | AAT Bioquest | 200 Tests | EUR 262.8 |
) spacer 0.8mm (2) |
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| EVS3100-SP-0.8 | Consort | ea | EUR 48 |
) spacer 1.5mm (2) |
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| EVS3100-SP-1.5 | Consort | ea | EUR 48 |
spacer 0.8mm (2) |
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| EVS3200-SP-0.8 | Consort | ea | EUR 57.6 |
spacer 1.5mm (2) |
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| EVS3200-SP-1.5 | Consort | ea | EUR 57.6 |
spacer 0.8mm (2) |
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| EVS3300-SP-0.8 | Consort | ea | EUR 57.6 |
spacer 1.5mm (2) |
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| EVS3300-SP-1.5 | Consort | ea | EUR 57.6 |
 Magainin Spacer Peptide |
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| 5-01496 | CHI Scientific | 4 x 1mg | Ask for price |
 CMK |
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| A3319-10 | ApexBio | 10 mg | EUR 602.4 |
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Description: CMK is a RSK2 kinase inhibitor. |
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CMK |
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| A3319-25 | ApexBio | 25 mg | EUR 841.2 |
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Description: CMK is a RSK2 kinase inhibitor. |
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CMK |
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| A3319-5 | ApexBio | 5 mg | EUR 385.2 |
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Description: CMK is a RSK2 kinase inhibitor. |
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CMK |
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| A3319-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 316.8 |
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Description: CMK is a RSK2 kinase inhibitor. |
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CMK |
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| HY-52101 | MedChemExpress | 2mg | EUR 224.4 |
 Canadian Pesticide Kit Containing All 6 Mixes |
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| CAN-CAN-KIT | Scientific Laboratory Supplies | 1ML | EUR 758.1 |
 5-FAM |
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| 20-abx095073 | Abbexa |
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 6-FAM |
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| HY-66021 | MedChemExpress | 500mg | EUR 374.4 |
5-FAM |
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| HY-66022 | MedChemExpress | 10mM/1mL | EUR 151.2 |
 FAM-cAMP |
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| 60200 | BPS Bioscience | 100 µl | EUR 245 |
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Description: The fluorogenic PDE substrate FAM-cAMP is used for cAMP-dependent phosphodiesterase assays. It has been used in activity assays for PDE1, PDE2, PDE3, PDE4, PDE7, PDE8, PDE10 and PDE11. |
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 FAM-cGMP |
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| 60201 | BPS Bioscience | 100 µl | EUR 235 |
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Description: The fluorogenic PDE substrate FAM-cGMP is used for cGMP-dependent phosphodiesterase assays. It has been used in activity assays for PDE5, PDE6 and PDE9. |
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 Cell Meter™ Cell Viability Assay Kit *Green Fluorescence* |
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| 22786 | AAT Bioquest | 500 Tests | EUR 262.8 |
 Cell Meter™ TUNEL Apoptosis Assay Kit *Green Fluorescence* |
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| 22849 | AAT Bioquest | 50 Tests | EUR 367.2 |
 Amplite™ MMP-3 Activity Assay Kit *Green Fluorescence* |
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| 13512 | AAT Bioquest | 100 Tests | EUR 367.2 |
 Amplite™ Fluorimetric HDAC Activity Assay Kit *Green Fluorescence* |
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| 13601 | AAT Bioquest | 200 Tests | EUR 262.8 |
 Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence* |
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| 12601 | AAT Bioquest | 500 Tests | EUR 367.2 |
 Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence* |
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| 11953 | AAT Bioquest | 500 Tests | EUR 262.8 |
, Trial Size) OxiSelect Intracellular ROS Assay Kit (Green Fluorescence), Trial Size |
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| STA-342-T | Cell Biolabs | 20 assays | EUR 414 |
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Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader. |
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) OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence) |
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| STA-347 | Cell Biolabs | 96 assays | EUR 734.4 |
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Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF. |
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OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence) |
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| STA-347-5 | Cell Biolabs | 5 x 96 assays | EUR 2913.6 |
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Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF. |
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, Green Fluorescence) EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence |
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| K452-100 | Biovision | each | EUR 588 |
, Green Fluorescence) EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence |
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| K453-100 | Biovision | each | EUR 588 |
, Green Fluorescence) EZClick? Myristoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence |
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| K497-100 | Biovision | each | EUR 588 |
 aluminum spacer) Hellma(R) aluminum spacer |
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| Z801135-1EA | Scientific Laboratory Supplies | EACH | EUR 65.66 |
Hellma(R) aluminum spacer |
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| Z801240-1EA | Scientific Laboratory Supplies | EACH | EUR 69.2 |
Hellma(R) aluminum spacer |
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| Z801356-1EA | Scientific Laboratory Supplies | EACH | EUR 69.2 |
 Amplite™ Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence* |
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| 5524 | AAT Bioquest | 200 Tests | EUR 262.8 |
 Cell Navigator™ Fluorimetric Lipid Droplet Assay Kit *Green Fluorescence* |
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| 22730 | AAT Bioquest | 200 Tests | EUR 262.8 |
 Amplite™ Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence* |
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| 13456 | AAT Bioquest | 100 tests | EUR 367.2 |
 Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence* |
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| 13500 | AAT Bioquest | 500 Tests | EUR 262.8 |
 Amplite™ Fluorimetric Caspase 3/7 Assay Kit *Green Fluorescence* |
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| 13503 | AAT Bioquest | 500 Tests | EUR 367.2 |
 Amplite™ Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence* |
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| 13510 | AAT Bioquest | 100 Tests | EUR 367.2 |
