bt3technologies

Activated-carbon-filled agarose hydrogel as a natural medium for seed germination and seedling growth

An activated-carbon-filled agarose (Agar-AC) hydrogel containing numerous concentrations of activated carbon (AC) was efficiently fabricated by a easy solvent solid approach. In comparison with pure agarose hydrogels, Agar-AC hydrogels exhibited glorious mechanical properties, good thermal stability and a extremely developed pore construction. The Agar-AC hydrogels additionally confirmed a sure diploma of enchancment in water retention efficiency, whereas their swelling ratio decreased with the addition of AC.
The incorporation of AC didn’t affect the crystallinity of the agarose hydrogel, and no chemical modification occurred in keeping with XRD and FTIR. In rapeseed seed germination experiments, the expansion indexes of rapeseed, together with the germination proportion, root size, stem size, recent weight and dry weight, had been enhanced by the incorporation of an appropriate quantity of AC. These outcomes indicated that AC has nice potential to boost the properties of agarose hydrogels and enhance seed germination and plant development, which suggests that Agar-AC hydrogels can be utilized as pure supplies for agricultural functions.

Impact of amine size within the interference of the multipoint covalent immobilization of enzymes on glyoxyl agarose beads

Trypsin, chymotrypsin, penicillin G acylase and ficin extract have been stabilized by immobilization on glyoxyl agarose, including completely different aliphatic compounds bearing a main amine group throughout the immobilization: ethyl amine, butyl amine, hexyl amine (at concentrations starting from Zero to 20 mM) and octyl amine (from Zero to 10 mM) to research their results on the immobilized enzyme stability. As anticipated, the presence of amines lowered the depth of the enzyme-support multipoint covalent attachment, and due to this fact the enzyme stability.
Nevertheless, it’s clear that this impact is larger utilizing octyl amine for all enzymes (in some circumstances the enzyme immobilized within the presence of 10 mM octyl amine was virtually inactivated whereas the reference stored over 50% of the preliminary exercise). This manner, plainly crucial impact of the presence of aminated compounds got here from the technology of steric hindrances to the enzyme/help multi-reaction promoted by the ammines which can be interacting with the aldehyde teams.
In some situations, simply 1 mM of aminated compounds is sufficient to tremendously lower enzyme stability. The outcomes recommended that, if the composition of the enzyme extract is unknown, to eradicate small aminated compounds could also be needed to maximise the enzyme-support response.

Hydrogen Bond Interpenetrated Agarose/PVA Community: A Extremely Ionic Conductive and Flame-Retardant Gel Polymer Electrolyte

The gel polymer electrolyte (GPE) is the important thing to assembling high-performance solid-state supercapacitors (SSCs). The industrial poly(vinyl alcohol) (PVA) GPE has developed a status because of low ionic conductivity endowed by its excessive crystallinity and poor water retention capability. On this work, density useful concept (DFT) calculations first revealed that the excessive crystallinity of PVA could be tremendously disrupted by forming hydrogen bonds with pure agarose macromolecules.
The hydrogen bond interpenetrated three-dimensional agarose/PVA community gives excessive water retention and huge quantities of channels for motion of Li+ on hydroxyl oxygen atoms. So, an optimized formation of the Li-O coordinate bond (gLi-O(r) = 8.78) and improved diffusion coefficient of Li+ (DLi+) (71 × 10-6 cm2 s-1) had been obtained within the agarose/PVA mannequin.
When assembled into SSCs, agarose/PVA-GPE with 2 M LiOAc (AP-GPE) reveals an excellent particular capacitance (697.22 mF cm-2 at 5 mA cm-2). The excessive water retention of agarose and huge quantities of -OH teams within the agarose macromolecular can generate H2O by dehydration response, lowering the flammability of PVA and tremendously enhancing the protection of SSCs.

Agarose-resolvable InDel markers primarily based on entire genome re-sequencing in cucumber

Insertion and Deletion (InDel) are widespread options in genomes and are related to genetic variation. The entire-genome re-sequencing knowledge from two dad and mom (X1 and X2) of the elite cucumber (Cucumis sativus) hybrid selection Lvmei No.1 was used for genome-wide InDel polymorphisms evaluation. Obtained sequence reads had been mapped to the genome reference sequence of Chinese language recent market sort inbred line ‘9930’ and gaps conforming to InDel had been pinpointed.
Additional, the extent of cross-parents polymorphism amongst 5 pairs of cucumber breeding dad and mom and their corresponding hybrid varieties had been used for evaluating hybrid seeds purity take a look at effectivity of InDel markers. A panel of 48 cucumber breeding traces was utilized for PCR amplification versatility and phylogenetic evaluation of those markers. In complete, 10,470 candidate InDel markers had been recognized for X1 and X2. Amongst these, 385 markers with greater than 30 nucleotide distinction had been arbitrary chosen. These markers had been chosen for experimental resolvability by electrophoresis on an Agarose gel.
2 hundred and eleven (211) accounting for 54.81% of markers may very well be validated as single and clear polymorphic sample whereas 174 (45.19%) confirmed unclear or monomorphic genetic bands between X1 and X2. Cross-parents polymorphism analysis recorded 68 (32.23%) of those markers, which had been designated as cross-parents transferable (CPT) InDel markers.
Apparently, the marker InDel114 offered experimental transferability between cucumber and melon. A panel of 48 cucumber breeding traces together with dad and mom of Lvmei No. 1 subjected to PCR amplification versatility utilizing CPT InDel markers efficiently clustered them into fruit and customary cucumber varieties primarily based on phylogenetic evaluation. It’s price noting that 16 of those markers had been predominately related to enzymatic actions in cucumber. These agarose-based InDel markers may represent a useful useful resource for hybrid seeds purity testing, germplasm classification and marker-assisted breeding in cucumber.
bt3technologies
bt3technologies

Single step immobilization of CMCase inside agarose gel matrix: Kinetics and thermodynamic research

Within the present research, CMCase from Bacillus licheniformis KIBGE-IB2 was immobilized throughout the matrix of agarose gel by entrapment approach. Most immobilization yield (%) of the enzyme was obtained when 2.0 % agarose was used. The activation vitality (Ea) of the enzyme elevated from 16.38 to 44.08 kJ mol-1 after immobilization.
Thermodynamic parameters reminiscent of activation vitality of deactivation (ΔGd), enthalpy (ΔHd) and entropy (ΔSd) of deactivation, deactivation fee fixed (Okd), half-life (thalf), D-value and z-value had been calculated for native/free and immobilized CMCase. The utmost response fee (Vmax) of the native enzyme was discovered to be 8319.47 U ml-1 min-1, which lowered to 7218.1 U ml-1 min-1after immobilization course of.
Nevertheless, the Michaelis-Menten fixed (Okm) worth of the enzyme elevated from 1.236 to 2.769 mg ml-1 min-1 after immobilization. Immobilized enzyme inside agarose gel matrix help could be reuse as much as eight response cycles. Broad stability profile and improved catalytic properties of the immobilized CMCase indicated that this enzyme is usually a believable candidate for use in numerous industrial processes.

FAM-Spacer-Phe-CMK FLISP™ Assay Kit

KF17323 100 Tests
EUR 834

FAM-Leu-CMK FLISP™ Assay Kit

KF17317 100 Tests
EUR 834

FAM-Phe-CMK FLISP™ Assay Kit

KF17314 25 Tests
EUR 349.2

FAM-Phe-CMK FLISP™ Assay Kit

KF17315 100 Tests
EUR 834

FAM-Lue-CMK FLISP™ Assay Kit

KF17316 25 Tests
EUR 349.2

SR-101-Leu-CMK FLISP™ Assay Kit

KF17321 100 Tests
EUR 834

FAM-Leu-CMK Serine Protease Assay Kit

abx299011-25tests 25 tests
EUR 679.2

SR-101-Phe-CMK FLISP™ Assay Kit

KF17318 25 Tests
EUR 349.2

SR-101-Phe-CMK FLISP™ Assay Kit

KF17319 100 Tests
EUR 834

FAM-Phe-CMK Serine Protease Assay Kit

abx299010-25tests 25 tests
EUR 679.2

FAM-Leu-DAP Serine Protease Assay Kit

abx299012-25tests 25 tests
EUR 679.2

OPTIONAL GREEN FILTER (572NM)

GDBL-GREEN 1/pk
EUR 1262.4
Description: Lab Equipment; Axygen Branded EQ

Polycaspase Assay Kit, green

KF17200 25 Tests
EUR 364.8

Malachite Green Phosphate Assay Kit

abx298878-100Assays 100 Assays
EUR 453.6

Caspase 1 Assay Kit, green

KF17201 25 Tests
EUR 390

Caspase 2 Assay Kit, green

KF17202 25 Tests
EUR 364.8

Caspase 6 Assay Kit, green

KF17204 25 Tests
EUR 364.8

Caspase 8 Assay Kit, green

KF17205 25 Tests
EUR 364.8

Caspase 9 Assay Kit, green

KF17206 25 Tests
EUR 364.8

Caspase 10 Assay Kit, green

KF17207 25 Tests
EUR 364.8

Caspase 13 Assay Kit, green

KF17208 25 Tests
EUR 364.8

Malachite Green Phosphate Assay Kit

POMG-25H 2500
EUR 330
Description: High-throµghput phosphate assay using malachite green method at 620nm. Kit size: 2500 tests. Detection limit: 0.02 µM. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

Malachite Green Phosphate Assay Kit

Z5030012 2,500 assays
EUR 410.4
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Malachite Green Phosphate Assay kit

65-POMG-25H 2500 tests
EUR 333

FAM-Phe-DAP Serine Protease Assay Kit

abx299013-25tests 25 tests
EUR 698.4

FAM-cAMP PDE IV substrate *Green Fluorescence*

13602 0.5 umol
EUR 367.2

FAM-cGMP PDE V substrate *Green Fluorescence*

13604 0.5 umol
EUR 367.2

Caspase 3 & 7 Assay Kit, green

KF17203 25 Tests
EUR 364.8

EZCell? Phagocytosis Assay Kit (Green Zymosan)

K397-100
EUR 516

Morpheus HT-96 Green Screen

M-MD1-47-GREEN 96 x 1 ml ml
EUR 280
Description: Morpheus HT-96 Green Screen

Pesticide Kit Containing All 10 Multi-Compound Mixes

SPXPR-KIT 1ML
EUR 1923.18

Puck Spacer

M-CBP-PSP1 1 UNIT
EUR 133
Description: Puck Spacer

Styrofoam Spacer

M-CP-111-009S 1 UNIT
EUR 6
Description: Styrofoam Spacer

EZCell? Phagocytosis Assay Kit (Green E. coli)

K963-100
EUR 516

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)

STA-342 96 assays
EUR 762
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)

STA-342-5 5 x 96 assays
EUR 3058.8
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect Cellular Antioxidant Assay Kit (Green Fluorescence)

STA-349 192 assays
EUR 644.4
Description: The OxiSelect Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells.  Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested.  After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator.  The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF.  The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.

Cell Meter™ Autophagy Assay Kit *Green Fluorescence*

23002 200 Tests
EUR 367.2

Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*

11401 200 Tests
EUR 262.8

Amplite™ Fluorimetric Glutathione Assay Kit *Green Fluorescence*

10055 200 Tests
EUR 262.8

spacer 0.8mm (2)

EVS3100-SP-0.8 ea
EUR 48

spacer 1.5mm (2)

EVS3100-SP-1.5 ea
EUR 48

spacer 0.8mm (2)

EVS3200-SP-0.8 ea
EUR 57.6

spacer 1.5mm (2)

EVS3200-SP-1.5 ea
EUR 57.6

spacer 0.8mm (2)

EVS3300-SP-0.8 ea
EUR 57.6

spacer 1.5mm (2)

EVS3300-SP-1.5 ea
EUR 57.6

Magainin Spacer Peptide

5-01496 4 x 1mg Ask for price

Canadian Pesticide Kit Containing All 6 Mixes

CAN-CAN-KIT 1ML
EUR 758.1

CMK

A3319-10 10 mg
EUR 602.4
Description: CMK is a RSK2 kinase inhibitor.

CMK

A3319-25 25 mg
EUR 841.2
Description: CMK is a RSK2 kinase inhibitor.

CMK

A3319-5 5 mg
EUR 385.2
Description: CMK is a RSK2 kinase inhibitor.

CMK

A3319-5.1 10 mM (in 1mL DMSO)
EUR 316.8
Description: CMK is a RSK2 kinase inhibitor.

CMK

HY-52101 2mg
EUR 224.4

Cell Meter™ Cell Viability Assay Kit *Green Fluorescence*

22786 500 Tests
EUR 262.8

Cell Meter™ TUNEL Apoptosis Assay Kit *Green Fluorescence*

22849 50 Tests
EUR 367.2

Amplite™ MMP-3 Activity Assay Kit *Green Fluorescence*

13512 100 Tests
EUR 367.2

Amplite™ Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*

13601 200 Tests
EUR 262.8

Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*

12601 500 Tests
EUR 367.2

Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*

11953 500 Tests
EUR 262.8

EZClick? Myristoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K497-100
EUR 588

EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K453-100
EUR 588

EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K452-100
EUR 588

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence), Trial Size

STA-342-T 20 assays
EUR 414
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)

STA-347 96 assays
EUR 734.4
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)

STA-347-5 5 x 96 assays
EUR 2913.6
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

5-FAM

20-abx095073
  • EUR 627.60
  • EUR 444.00
  • EUR 1528.80
  • 1 g
  • 500 mg
  • 5 g

6-FAM

HY-66021 500mg
EUR 374.4

5-FAM

HY-66022 10mM/1mL
EUR 151.2

Hellma(R) aluminum spacer

Z801135-1EA EACH
EUR 65.66

Hellma(R) aluminum spacer

Z801240-1EA EACH
EUR 69.2

Hellma(R) aluminum spacer

Z801356-1EA EACH
EUR 69.2

Amplite™ Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*

5524 200 Tests
EUR 262.8

Cell Navigator™ Fluorimetric Lipid Droplet Assay Kit *Green Fluorescence*

22730 200 Tests
EUR 262.8

Amplite™ Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

13456 100 tests
EUR 367.2

Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*

13500 500 Tests
EUR 262.8

Amplite™ Fluorimetric Caspase 3/7 Assay Kit *Green Fluorescence*

13503 500 Tests
EUR 367.2

Amplite™ Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*

13510 100 Tests
EUR 367.2

Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Green Fluorescence*

16315 100 Tests
EUR 420

EZClick? Global Protein Synthesis Assay Kit (FACS/Microscopy), Green Fluorescence

K459-100
EUR 660

Cell Meter™ Cellular Senescence Activity Assay Kit *Green Fluorescence*

23005 100 Tests
EUR 334